Definition
The formalin test refers to the quantification of characteristic nociceptive behaviors that occur in response to subcutaneous (s.c.) or intradermal injection of a dilute solution of formaldehyde in 0.9 % saline, typically into the dorsal or plantar hindpaw of rodents.
Characteristics
The formalin test was originally described by Dubuisson and Dennis (1977) using 50 μl of 5 % formalin injected s.c. into the dorsal surface of one forepaw in rats and cats. “Five percent formalin” consisted of 1 ml of saturated formaldehyde (37 %) in water + 19 ml 0.9 % saline (i.e., 1.85 % formaldehyde). It is now more common to inject between 0.2 % and 5 % formalin into the dorsal or plantar hindpaw, using 20–50 μl in rats or 10–25 μl in mice. Another common site is the lateral aspect of the muzzle, or the temporomandibular joint, in rats, as a model of orofacial pain (Clavelou et al. 1995). The hindpaw has replaced the forepaw...
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References
Abbott, F. V., Franklin, K. B. J., & Westbrook, R. F. (1995). The formalin test: Scoring properties of the first and second phases of the pain response in rats. Pain, 60, 91–102.
Bráz, J. M., & Basbaum, A. I. (2010). Differential ATF3 expression in dorsal root ganglion neurons reveals the profile of primary afferents engaged by diverse chemical stimuli. Pain, 150, 290–301.
Clavelou, P., Dallel, R., Orliaguet, T., Woda, A., & Raboisson, P. (1995). The orofacial formalin test in rats: Effects of different formalin concentrations. Pain, 62, 295–301.
Coderre, T. J. (2001). Noxious stimulus-induced plasticity in spinal cord dorsal horn: Evidence and insights on mechanisms obtained using the formalin test. In M. M. Patterson & J. W. Grau (Eds.), Spinal cord plasticity: Alterations in reflex function (pp. 163–183). Boston: Kluwer Academic.
Coderre, T. J., Fundytus, M. E., McKenna, J. E., Dalal, S., & Melzack, R. (1993). The formalin test: A validation of the weighted-scored method of behavioral pain rating. Pain, 54, 43–50.
Dickenson, A. H., & Sullivan, A. F. (1987). Subcutaneous formalin-induced activity of dorsal horn neurones in the rat: Differential response to an intrathecal opioid administered pre or post formalin. Pain, 30, 349–360.
Dubuisson, D., & Dennis, S. G. (1977). The formalin test: A quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats. Pain, 4, 161–174.
Henry, J. L., Yashpal, K., Pitcher, G. M., & Coderre, T. J. (1999). Physiological evidence that the “Interphase” in the formalin test is due to active inhibition. Pain, 82, 57–63.
Hunskaar, S., Fasmer, O. B., & Hole, K. (1985). Formalin test in mice, a useful technique for evaluating mild analgesics. Journal of Neuroscience Methods, 14, 69–76.
Jourdan, D., Ardid, D., & Eschalier, A. (2001). Automated behavioural analysis in animal pain studies. Pharmacological Research, 43, 103–110.
Macpherson, L. J., Xiao, B., Kwan, K. Y., Petrus, M. J., Dubin, A. E., Hwang, S. W., Cravatt, B., Corey, D. P., & Patapoutian, A. (2007). An ion channel essential for sensing chemical damage. The Journal of Neuroscience, 27, 11412–11415.
McNamara, C. R., Mandel-Brehm, J., Bautista, D. M., Siemens, J., Deranian, K. L., Zhao, M., Hayward, N. J., Chong, J. A., Julius, D., Moran, M. M., & Fanger, C. M. (2007). TRPA1 mediates formalin-induced pain. Proceedings of National Academy of Sciences USA, 104, 13525–13530.
Puig, S., & Sorkin, L. S. (1995). Formalin-evoked activity in identified primary afferent fibres: Systemic lidocaine suppresses phase-2 activity. Pain, 64, 345–355.
Sawynok, J., & Liu, X. J. (2003). The formalin test: Characteristics and usefulness of the model. Reviews in Analgesia, 7, 145–163.
Sawynok, J., & Reid, A. R. (2003). Chronic intrathecal cannulas inhibit some and potentiate other behaviors elicited by formalin injection. Pain, 103, 7–9.
Shields, S. D., Cavanaugh, D. J., Lee, H., Anderson, D. J., & Basbaum, A. I. (2010). Pain behavior in the formalin test persists after ablation of the great majority of C-fiber nociceptors. Pain, 151, 422–429.
Taylor, B. K., Peterson, M. A., & Basbaum, A. I. (1995). Persistent cardiovascular and behavioral nociceptive responses to subcutaneous formalin require peripheral nerve input. The Journal of Neuroscience, 15, 7575–7584.
Tjølsen, A., Berge, O. G., Hunskaar, S., Rosland, J. H., & Hole, K. (1992). The formalin test: An evaluation of the method. Pain, 51, 5–17.
Wheeler-Aceto, H., & Cowan, A. (1991). Standardization of the rat paw formalin test for the evaluation of analgesics. Psychopharmacology, 104, 35–44.
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Coderre, T.J., Abbott, F.V., Sawynok, J. (2013). Formalin Test. In: Gebhart, G.F., Schmidt, R.F. (eds) Encyclopedia of Pain. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28753-4_1535
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DOI: https://doi.org/10.1007/978-3-642-28753-4_1535
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