This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bretschneider S, Eggeling C, Hell SW. Breaking the diffraction barrier in fluorescence microscopy by optical shelving. Phys Rev Lett. 2007;98:218103.
Diaspro A, editor. Optical fluorescence microscopy: from the spectral to the nano dimension. Heidelberg: Springer; 2010a. p. 1–244.
Diaspro A, editor. Nanoscopy and multidimensional optical fluorescence microscopy. Baco Raton: Taylor &Francis Group/A Chapman & Hall Book/CRC Press; 2010b. p. 1–448.
Fischer J, von Freymann G, Wegener M. The materials challenge in diffraction-unlimited direct-laser-writing optical lithography. Adv Mater. 2010;22:3578–82.
Galiani S, Harke B, Vicidomini G, et al. Strategies to maximize the performance of a STED microscope. Opt Express. 2012;20:7362–74.
Grotjohann T, Testa I, Leutenegger M, et al. Diffraction-unlimited all-optical imaging and writing with a photochromic GFP. Nature. 2011;478:204–8.
Harke B. 3D STED microscopy with pulsed and continuous wave lasers. PhD-Thesis, Niedersächsische Staats-und Universitätsbibliothek Göttingen; 2008.
Harke B, Keller J, Ullal CK, et al. Resolution scaling in STED microscopy. Opt Express. 2008a;16:4154–62.
Harke B, Ullal CK, Keller J, Hell SW. Three-dimensional nanoscopy of colloidal crystals. Nano Lett. 2008b;8:1309–13.
Harke B, Bianchini P, Brandi F, Diaspro A. Photopolymerization inhibition dynamics for sub−diffraction direct laser writing lithography. Chemphyschem. 2012. doi:10.1002/cphc.201200006.
Hell SW. Toward fluorescence nanoscopy. Nat Biotechnol. 2003;21:1347–55.
Hell SW. Strategy for far-field optical imaging and writing without diffraction limit. Phys Lett A. 2004;326:140–5.
Hell SW, Wichmann J. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt Lett. 1994;19:780–2.
Hofmann M, Eggeling C, Jakobs S, Hell SW. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. Proc Natl Acad Sci USA. 2005;102:17565.
Pawley JB, editor. Handbook of biological confocal microscopy. 3rd ed. New York: Springer Science; 2006.
Vicidomini G, Moneron G, Han KY, et al. Sharper low-power STED nanoscopy by time gating. Nat Methods. 2011;8:571–3.
Westphal V, Hell SW. Nanoscale resolution in the focal plane of an optical microscope. Phys Rev Lett. 2005;94:143903.
Wildanger D, Rittweger E, Kastrup L, et al. STED microscopy with a supercontinuum laser source. Opt Express. 2008;16:9614–21.
Wildanger D, Medda R, Kastrup L, Hell S. A compact STED microscope providing 3D nanoscale resolution. J Microsc. 2009;236:35–43.
Willig KI, Harke B, Medda R, Hell SW. STED microscopy with continuous wave beams. Nat Methods. 2007;4:915–8.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 European Biophysical Societies' Association (EBSA)
About this entry
Cite this entry
Harke, B., Bianchini, P., Vicidomini, G., Galiani, S., Diaspro, A. (2013). Stimulated Emission Depletion (STED) Microscopy. In: Roberts, G.C.K. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16712-6_835
Download citation
DOI: https://doi.org/10.1007/978-3-642-16712-6_835
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-16711-9
Online ISBN: 978-3-642-16712-6
eBook Packages: Biomedical and Life SciencesReference Module Biomedical and Life Sciences