Abstract
The measurement of fluorescence in planar chromatography is a sensitive method for the determination of fluorescing substances [1–9]. Fluorescence emission provides more selectivity and increased sensitivity compared to UV–visible absorption. Molecular fluorescence can be described as the immediate emission of energy from a molecule after irradiation. During absorption (this process is called radiative excitation), a molecule is excited from a lower to a higher electronic state by absorption of a photon. During de-excitation, a molecule emits a photon and changes from a higher to a lower electronic state. A transition between states of the same multiplicity is known as fluorescence and a transition between states of different multiplicities is called phosphorescence.
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Spangenberg, B., Poole, C.F., Weins, C. (2010). Fluorescence in TLC Layers. In: Quantitative Thin-Layer Chromatography. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-10729-0_11
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DOI: https://doi.org/10.1007/978-3-642-10729-0_11
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