Abstract
The green alga Chlorella vulgaris has the ability to bind high amounts of uranium(VI) in the pH range from 3 to 6 and to a lesser extend at higher pH values. The uranium removal is almost complete at pH 4.4 and 6 by metabolic active and inactive cells under the given experimental conditions. Laser-induced fluorescence spectroscopy was used for the characterization of uranyl species formed in solutions and biomass. Fluorescence spectroscopic investigations indicate differences of the formed algal uranyl complexes in dependence of the metabolic activity of cells and the uranyl speciation. Scanning electron microscopy demonstrates that the algal cell wall is involved in the binding of U(VI).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bernhard G, Geipel G, Brendler V, Nitsche H (1996) Speciation of uranium in seepage waters of a mine tailing pile studied by time-resolved laser-induced fluorescence spectroscopy (TRLFS). Radiochim Acta 74: 87-91
Brachmann A, Geipel G, Bernhard G, Nitsche H (2002) Study of uranyl(VI) malonate complexation by time-resolved laser-induced fluorescence spectroscopy (TRLFS). Radiochim Acta 90: 147-153
Eliet V, Bidoglio G, Omenetto N, Parma L, Grenthe I (1995) Characterisation of hydroxide complexes of uranium(VI) by time-resolved laser-induced fluorescence spectroscopy. J Chem Soc Faraday Trans 91: 2275-2285
Esser K (2000) Kryptogamen. Springer: Berlin, Heidelberg, New York
Geipel G, Berhard G, Rutsch M, Brendler V, Nitsche H (2000) Spectroscopic properties of uranium(VI) minerals studied by time-resolved laser-induced fluorescence spectroscopy (TRLFS). Radiochim Acta 88: 757-762
Geipel G, Brachmann A, Brendler V, Bernhard G, Nitsche H (1996) Uranium(VI) sulfate complexation studies by time-resolved laser-induced fluorescence spectroscopy. Radiochim Acta 75: 199-204
Guillaumont R, Fanghönel T, Fuger J, Grenthe I, Neck V, Palmer DA, Rand MH (2003) Update on the chemical thermodynamics of uranium, neptunium, plutonium, americium and technetium. Chemical Thermodynamics 5 (OECD Nuclear Energy Agency, ed.) Elsevier, Amsterdam
Günther A, Bernhard G, Geipel G, Reich T, Roβberg A, Nitsche H (2003) Uranium speciation in plants. Radiochim Acta 91: 319-328
Günther A, Geipel G, Bernhard G (2007) Complex formation of uranium(VI) with amino acids L-glycine and L-cysteine: A fluorescence emission and UV-Vis absorption study. Polyhedron 26: 59-65
Günther A, Raff J, Geipel G, Bernhard G (2008) Spectroscopic investigations of U(VI) species sorbed by the green algae Chlorella vulgaris. Biometals 21 (3): 333-341
Kuhl A, Lorenzen H (1964) Handling and culturing of Chlorella. In: Methods in cell physiology, vol. I: 159–187, Academic Press: New York
Panak PJ, Raff J, Selenska-Pobell S, Geipel G, Bernhard G, Nitsche H (2000) Complex formation of (UVI) with Bacillus-isolates from a uranium mining waste pile. Radiochim Acta 88: 71-76
Sachs S, Brendler V, Geipel G (2007) Uranium(VI) complexation by humic acid under neutral pH conditions studied by laser-induced fluorescence spectroscopy. Radiochim Acta 95: 103-110
Schmidt PF, Balk LJ, Blaschke R, Bröcker W, Demm E, Engel L, Göcke R, Hantsche H, Hauert R, Krefting ER, Müller T, Raith H, roth M, Woodtli J (1994) Praxis der Rasterelektronenmikroskopie und Mikrobereichsanalyse. Kontakt & Studium Band 444, Expert Verlag
Wolery TJ (1992) EQ 3/6 a software package for the geochemical modeling of aqueous systems, Report UCRL-MA-110662 part1, Lawrence Livermore National Laboratory, California, USA
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Vogel, M., Günther, A., Raff, J., Bernhard, G. (2008). Microscopic and spectroscopic investigation of U(VI) interaction with monocellular green algae. In: Merkel, B.J., Hasche-Berger, A. (eds) Uranium, Mining and Hydrogeology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-87746-2_89
Download citation
DOI: https://doi.org/10.1007/978-3-540-87746-2_89
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-87745-5
Online ISBN: 978-3-540-87746-2
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)