Skip to main content

Microscopic and spectroscopic investigation of U(VI) interaction with monocellular green algae

  • Chapter
Uranium, Mining and Hydrogeology
  • 106 Accesses

Abstract

The green alga Chlorella vulgaris has the ability to bind high amounts of uranium(VI) in the pH range from 3 to 6 and to a lesser extend at higher pH values. The uranium removal is almost complete at pH 4.4 and 6 by metabolic active and inactive cells under the given experimental conditions. Laser-induced fluorescence spectroscopy was used for the characterization of uranyl species formed in solutions and biomass. Fluorescence spectroscopic investigations indicate differences of the formed algal uranyl complexes in dependence of the metabolic activity of cells and the uranyl speciation. Scanning electron microscopy demonstrates that the algal cell wall is involved in the binding of U(VI).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 389.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 499.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bernhard G, Geipel G, Brendler V, Nitsche H (1996) Speciation of uranium in seepage waters of a mine tailing pile studied by time-resolved laser-induced fluorescence spectroscopy (TRLFS). Radiochim Acta 74: 87-91

    Google Scholar 

  • Brachmann A, Geipel G, Bernhard G, Nitsche H (2002) Study of uranyl(VI) malonate complexation by time-resolved laser-induced fluorescence spectroscopy (TRLFS). Radiochim Acta 90: 147-153

    Article  Google Scholar 

  • Eliet V, Bidoglio G, Omenetto N, Parma L, Grenthe I (1995) Characterisation of hydroxide complexes of uranium(VI) by time-resolved laser-induced fluorescence spectroscopy. J Chem Soc Faraday Trans 91: 2275-2285

    Article  Google Scholar 

  • Esser K (2000) Kryptogamen. Springer: Berlin, Heidelberg, New York

    Book  Google Scholar 

  • Geipel G, Berhard G, Rutsch M, Brendler V, Nitsche H (2000) Spectroscopic properties of uranium(VI) minerals studied by time-resolved laser-induced fluorescence spectroscopy (TRLFS). Radiochim Acta 88: 757-762

    Article  Google Scholar 

  • Geipel G, Brachmann A, Brendler V, Bernhard G, Nitsche H (1996) Uranium(VI) sulfate complexation studies by time-resolved laser-induced fluorescence spectroscopy. Radiochim Acta 75: 199-204

    Google Scholar 

  • Guillaumont R, Fanghönel T, Fuger J, Grenthe I, Neck V, Palmer DA, Rand MH (2003) Update on the chemical thermodynamics of uranium, neptunium, plutonium, americium and technetium. Chemical Thermodynamics 5 (OECD Nuclear Energy Agency, ed.) Elsevier, Amsterdam

    Google Scholar 

  • Günther A, Bernhard G, Geipel G, Reich T, Roβberg A, Nitsche H (2003) Uranium speciation in plants. Radiochim Acta 91: 319-328

    Article  Google Scholar 

  • Günther A, Geipel G, Bernhard G (2007) Complex formation of uranium(VI) with amino acids L-glycine and L-cysteine: A fluorescence emission and UV-Vis absorption study. Polyhedron 26: 59-65

    Article  Google Scholar 

  • Günther A, Raff J, Geipel G, Bernhard G (2008) Spectroscopic investigations of U(VI) species sorbed by the green algae Chlorella vulgaris. Biometals 21 (3): 333-341

    Article  Google Scholar 

  • Kuhl A, Lorenzen H (1964) Handling and culturing of Chlorella. In: Methods in cell physiology, vol. I: 159–187, Academic Press: New York

    Google Scholar 

  • Panak PJ, Raff J, Selenska-Pobell S, Geipel G, Bernhard G, Nitsche H (2000) Complex formation of (UVI) with Bacillus-isolates from a uranium mining waste pile. Radiochim Acta 88: 71-76

    Article  Google Scholar 

  • Sachs S, Brendler V, Geipel G (2007) Uranium(VI) complexation by humic acid under neutral pH conditions studied by laser-induced fluorescence spectroscopy. Radiochim Acta 95: 103-110

    Article  Google Scholar 

  • Schmidt PF, Balk LJ, Blaschke R, Bröcker W, Demm E, Engel L, Göcke R, Hantsche H, Hauert R, Krefting ER, Müller T, Raith H, roth M, Woodtli J (1994) Praxis der Rasterelektronenmikroskopie und Mikrobereichsanalyse. Kontakt & Studium Band 444, Expert Verlag

    Google Scholar 

  • Wolery TJ (1992) EQ 3/6 a software package for the geochemical modeling of aqueous systems, Report UCRL-MA-110662 part1, Lawrence Livermore National Laboratory, California, USA

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Vogel, M., Günther, A., Raff, J., Bernhard, G. (2008). Microscopic and spectroscopic investigation of U(VI) interaction with monocellular green algae. In: Merkel, B.J., Hasche-Berger, A. (eds) Uranium, Mining and Hydrogeology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-87746-2_89

Download citation

Publish with us

Policies and ethics