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Advances in Plant Ecophysiology Techniques pp 195–213Cite as

Flow Cytometric Measurement of Different Physiological Parameters

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Abstract

A variety of physiological parameters involved in signal transduction pathways, enzymatic activities, ATP production, and many other physiological processes can be analyzed by flow cytometry. Parameters as intracellular pH, membrane potential, calcium concentration, reactive oxygen species (ROS) generation, or glutathione content can be estimated thanks to this technique. One of the principal advantages of flow cytometry is that it allows the measurement of these parameters in living and small cells, usually in real time during the physiological stimulation. Moreover, flow cytometry can detect not only the fluorescence of biochemically specific developed fluorescent dyes but also autofluorescence (such as the chlorophyll a fluorescence), which can be measured in parallel and can provide supplementary information about the physiological condition of the measured cells.

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Acknowledgement

This chapter is an update of ‘Coba de la Peña (2001) Use of flow cytometry to measure physiological parameters. In: Reigosa MJ (Ed), Handbook of Plant Ecophysiology Techniques. Kluwer Academic Publishers, The Netherlands, pp. 53–64.’

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Authors and Affiliations

  1. Dipartimento di Agraria, Facoltà di Agraria, Università Mediterranea di Reggio Calabria, Reggio Calabria, Italy

    Fabrizio Araniti

  2. Centro de Estudios Avanzados en Zonas Áridas (CEAZA), La Serena, Chile

    Teodoro Coba de la Peña

  3. Department of Plant Biology and Soil Science, University of Vigo, Vigo, Spain

    Adela M. Sánchez-Moreiras

Authors
  1. Fabrizio Araniti
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  2. Teodoro Coba de la Peña
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  3. Adela M. Sánchez-Moreiras
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Corresponding author

Correspondence to Adela M. Sánchez-Moreiras .

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Editors and Affiliations

  1. Department of Plant Biology and Soil Science, University of Vigo, Vigo, Spain

    Adela M. Sánchez-Moreiras

  2. Department of Plant Biology and Soil Science, University of Vigo, Vigo, Spain

    Manuel J. Reigosa

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Araniti, F., Coba de la Peña, T., Sánchez-Moreiras, A.M. (2018). Flow Cytometric Measurement of Different Physiological Parameters. In: Sánchez-Moreiras, A., Reigosa, M. (eds) Advances in Plant Ecophysiology Techniques. Springer, Cham. https://doi.org/10.1007/978-3-319-93233-0_11

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