Skip to main content
SpringerLink
Log in
Book cover

Springer Handbook of Odor pp 57–58Cite as

High Throughput Receptor Screening Assays

  • Chapter

  • 6670 Accesses

Part of the book series: Springer Handbooks ((SHB))

Abstract

The recent technical advances in functional expression of olfactory receptors (GlossaryTerm

OR

s) make full deorphanization of human OR repertoire a realistic objective. Such a global knowledge of the precise mechanisms of odorant/receptor pairings will represent a crucial step for the development of an accurate model of how human nose perceives its chemical environment. Beyond its interest for basic science, it will also lead to the development of industrial applications such as receptor-based odorant design, development of selective odor blockers or enhancers and represents therefore an interesting opportunity for players active in the field of flavors and fragrances. Here, we will describe and discuss a high-throughput screening approach that aims at the objective of human OR mass deorphanization. However, the completion of this ambitious task is not a prerequisite to the development of commercial applications. With the expanding number of deorphanized ORs, structure–activity relationship studies on OR responding to an odorant of interest has already started. Likewise, the use of the screening approach to identify either blockers for malodor-responding ORs or positive enhancers of fine fragrance-tuned ORs is underway. These different aspects will also be discussed. Finally, beyond the human ORs, other classes of human chemoreceptors for volatiles as well as animal chemoreceptors may also represent industrial opportunities that will be briefly reviewed.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   229.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   299.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Abbreviations

\(\upmu{}\mathrm{M}\) :

micromolar

3-D:

three-dimensional

AMP:

adenosine monophosphate

cAMP:

cyclic adenosine monophosphate

CNG:

cyclic nucleotide gated

DMSO:

dimethyl sulfoxide

DNA:

deoxyribonucleic acid

EMEM:

Eagle’s modified essential medium

FDSS:

functional drug screening system

fMRI:

functional magnetic resonance imaging

GFP:

green fluorescent protein

GPCR:

G protein coupled receptor

HEK:

human embryonic kidney cell

HTS:

high-throughput screening

IBMX:

isobutyl methyl xanthine

OBP:

odor binding protein

ODR:

odorant response

OR:

olfactory receptor

PCR:

polymerase chain reaction

PLC:

phospholipase C

SAR:

structure–activity relationship

TAAR:

trace amine associated receptor

UV:

ultraviolet

References

  1. L. Buck, R. Axel: A novel multigene family may encode odorant receptors: A molecular basis for odor recognition, Cell 65, 175187 (1991)

    Article  Google Scholar 

  2. B. Malnic, J. Hirono, T. Sato, L.B. Buck: Combinatorial receptor codes for odors, Cell 96, 713–723 (1999)

    Article  CAS  Google Scholar 

  3. E. Polak: Multiple profile-multiple receptor site model for vertebrate olfaction, J. Theor. Biol. 40, 469–484 (1973)

    Article  CAS  Google Scholar 

  4. A. Keller, H. Zhuang, Q. Chi, L.B. Vosshall, H. Matsunami: Genetic variation in a human odorant receptor alters odour perception, Nature 449, 468–472 (2007)

    Article  CAS  Google Scholar 

  5. J.D. Mainland, A. Keller, Y.R. Li, T. Zhou, C. Trimmer, L.L. Snyder, A.H. Moberly, K.A. Adipietro, W.L.L. Liu, H. Zhuang, S. Zhan, S.S. Lee, A. Lin, H. Matsunami: The missense of smell: Functional variability in human odorant receptor repertoire, Nat. Neurosci. 17(1), 114–120 (2014), doi:10.1038/nn.3598

    Article  CAS  Google Scholar 

  6. K.A. Adipietro, J.D. Mainland, H. Matsunami: Functional evolution of Mammalian odorant receptors, PLoS Genetics 8, e1002821 (2012)

    Article  CAS  Google Scholar 

  7. H. Hatt, J. Panten: Sandalwood aroma receptor, PCT Patent WO2008017604 (2008)

    Google Scholar 

  8. H. Saito, Q. Chi, H. Zhuang, H. Matsunami, J.D. Mainland: Odor coding by a Mammalian receptor repertoire, Sci. Signal 2, ra9 (2009)

    Article  Google Scholar 

  9. I. Menashe, T. Abaffy, Y. Hasin, S. Goshen, V. Yahalom, C.W. Luetje, D. Lancet: Genetic elucidation of human hyperosmia to isovaleric acid, PLoS Biology 5, 2462–2468 (2007)

    Article  CAS  Google Scholar 

  10. K. Schmiedeberg, E. Shirokova, H.P. Weber, B. Schilling, W. Meyerhof, D. Krautwurst: Structural determinants of odorant recognition by the human olfactory receptors OR1A1 and OR1A2, J. Struct. Biol. 159, 400–412 (2007)

    Article  CAS  Google Scholar 

  11. M. Spehr, G. Gisselmann, A. Poplawski, J.A. Riffell, C.H. Wetzel, R.K. Zimmer, H. Hatt: Identification of a testicular odorant receptor mediating human sperm chemotaxis, Science 299, 2054–2058 (2003)

    Article  CAS  Google Scholar 

  12. V. Matarazzo, O. Clot-Faybesse, B. Marcet, G. Guiraudie-Capraz, B. Atanasova, G. Devauchelle, M. Cerutti, P. Etievant, C. Ronin: Functional characterization of two human olfactory receptors expressed in the baculovirus Sf9 insect cell system, Chem. Senses 30, 195–207 (2005)

    Article  CAS  Google Scholar 

  13. G. Sanz, C. Schlegel, J.C. Pernollet, L. Briand: Comparison of odorant specificity of two human olfactory receptors from different phylogenetic classes and evidence for antagonism, Chem. Senses 30, 69–80 (2005)

    Article  CAS  Google Scholar 

  14. A. Mashukova, M. Spehr, H. Hatt, E.M. Neuhaus: β-Arrestin2-mediated internalization of mammalian odorant receptors, J. Neurosci. 26, 9902–9912 (2006)

    Article  CAS  Google Scholar 

  15. J.F. McRae, J.D. Mainland, S.R. Jaeger, K.A. Adipietro, H. Matsunami, R.D. Newcomb: Genetic variation in the odorant receptor OR2J3 is associated with the ability to detect the ‘‘grassy smelling’’ odor, cis-3-hexen-1-ol, Chem. Senses 37, 585–593 (2012)

    Article  CAS  Google Scholar 

  16. C.H. Wetzel, M. Oles, C. Wellerdieck, M. Kuczkowiak, G. Gisselmann, H. Hatt: Specificity and sensitivity of a human olfactory receptor functionally expressed in human embryonic kidney 293 cells and Xenopus laevis oocytes, J. Neurosci. 19, 7426–7433 (1999)

    CAS  Google Scholar 

  17. S.R. Jaeger, J.F. McRae, C.M. Bava, M.K. Beresford, D. Hunter, Y. Jia, S.L. Chheang, D. Jin, M. Peng, J.C. Gamble, K.R. Atkinson, L.G. Axten, A.G. Paisley, L. Tooman, B. Pineau, S.A. Rouse, R.D. Newcomb: A Mendelian trait for olfactory sensitivity affects odor experience and food selection, Curr. Biol. 23, 1601–1605 (2013)

    Article  CAS  Google Scholar 

  18. Y. Fujita, T. Takahashi, A. Suzuki, K. Kawashima, F. Nara, R. Koishi: Deorphanization of Dresden G protein-coupled receptor for an odorant receptor, J. Recept. Signal Transduct. Res. 27, 323–334 (2007)

    Article  CAS  Google Scholar 

  19. A. Kato, N. Saito, E. Wakisaka: Method for searching for malodor control agent, malodor control agent, and malodor control method, Patent US20130216492 (2013)

    Google Scholar 

  20. P. Chatelain, A. Veithen: Olfactory receptors involved in the perception of sweat carboxylic acids and the use thereof, Patent US20130336910 A1 (2014)

    Google Scholar 

  21. A. Veithen, M. Philippeau, F. Wilkin, P. Chatelain: OR5D3P, a pseudogene with a functional activity potential, Proc. ACHEMS, A58 (2009)

    Google Scholar 

  22. J.E. Amoore: Specific anosmia: A clue to the olfactory code, Nature 214, 1095–1098 (1967)

    Article  CAS  Google Scholar 

  23. J.E. Amoore: Specific anosmia and the concept of primary odors, Chem. Senses 2, 267–281 (1977)

    Article  CAS  Google Scholar 

  24. H. Hatt: Citrusal aroma receptors, WO 2008/017598 (2008)

    Google Scholar 

  25. A. Kato, N. Saito: Method for identifying a malodor inhibitor, Patent WO2012169644 (2012)

    Google Scholar 

  26. T. Olender, D. Lancet, D.W. Nebert: Update on the olfactory receptor (OR) gene superfamily, Hum. Genomics 3, 87–97 (2008)

    Article  CAS  Google Scholar 

  27. A. Matsui, Y. Go, Y. Niimura: Degeneration of olfactory receptor gene repertories in primates: No direct link to full trichromatic vision, Mol. Biol. Evol. 27, 1192–1200 (2010)

    Article  CAS  Google Scholar 

  28. I. Menashe, D. Lancet: Variations in the human olfactory receptor pathway, Cell. Mol. Life Sci. 63, 1485–1493 (2006)

    Article  CAS  Google Scholar 

  29. A. Veithen, F. Wilkin, M. Philippeau, P. Chatelain: Human olfaction: From the nose to receptors, Perfum. Flavorist 34, 36–44 (2009)

    CAS  Google Scholar 

  30. H. Matsunami: Functional expression of mammalian odorant receptors, Chem. Senses 30, i95–i96 (2005)

    Article  CAS  Google Scholar 

  31. P. Mombaerts: Genes and ligands for odorant, vomeronasal and taste receptors, Nat. Rev. Neurosci. 5, 263–278 (2004)

    Article  CAS  Google Scholar 

  32. H. Zhao, L. Ivic, J.M. Otaki, M. Hashimoto, K. Mikoshiba, S. Firestein: Functional expression of a mammalian odorant receptor, Science 279, 237–242 (1998)

    Article  CAS  Google Scholar 

  33. K. Touhara, S. Sengoku, K. Inaki, A. Tsuboi, J. Hirono, T. Sato, H. Sakano, T. Haga: Functional identification and reconstitution of an odorant receptor in single olfactory neurons, Proc. Natl. Acad. Sci. USA 96, 4040–4045 (1999)

    Article  CAS  Google Scholar 

  34. J.R. Murrell, D.D. Hunter: An olfactory sensory neuron line, odora, properly targets olfactory proteins and responds to odorants, J. Neurosci. 19, 8260–8270 (1999)

    CAS  Google Scholar 

  35. G. Levasseur, M.A. Persuy, D. Grebert, J.J. Remy, R. Salesse, E. Pajot-Augy: Ligand-specific dose-response of heterologously expressed olfactory receptors, Eur. J. Biochem. 270, 2905–2912 (2003)

    Article  CAS  Google Scholar 

  36. A.A. Gimelbrant, S.L. Haley, T.S. McClintock: Olfactory receptor trafficking involves conserved regulatory steps, J. Biol. Chem. 276, 7285–7290 (2001)

    Article  CAS  Google Scholar 

  37. D. Krautwurst, K.W. Yau, R.R. Reed: Identification of ligands for olfactory receptors by functional expression of a receptor library, Cell 95, 917–926 (1998)

    Article  CAS  Google Scholar 

  38. K. Kajiya, K. Inaki, M. Tanaka, T. Haga, H. Kataoka, K. Touhara: Molecular bases of odor discrimination: Reconstitution of olfactory receptors that recognize overlapping sets of odorants, J. Neurosci. 21, 6018–6025 (2001)

    CAS  Google Scholar 

  39. H. Saito, M. Kubota, R.W. Roberts, Q. Chi, H. Matsunami: RTP family members induce functional expression of mammalian odorant receptors, Cell 119, 679–691 (2004)

    Article  CAS  Google Scholar 

  40. E. Shirokova, K. Schmiedeberg, P. Bedner, H. Niessen, K. Willecke, J.D. Raguse, W. Meyerhof, D. Krautwurst: Identification of specific ligands for orphan olfactory receptors. G protein-dependent agonism and antagonism of odorants, J. Biol. Chem. 280, 11807–11815 (2005)

    Article  CAS  Google Scholar 

  41. K. Raming, J. Krieger, J. Strotmann, I. Boekhoff, S. Kubick, C. Baumstark, H. Breer: Cloning and expression of odorant receptors, Nature 361, 353–356 (1993)

    Article  CAS  Google Scholar 

  42. V. Matarazzo, N. Zsurger, J.C. Guillemot, O. Clot-Faybesse, J.M. Botto, C. Dal Farra, M. Crowe, J. Demaille, J.P. Vincent, J. Mazella, C. Ronin: Porcine odorant-binding protein selectively binds to a human olfactory receptor, Chem. Senses 27, 691–701 (2002)

    Article  CAS  Google Scholar 

  43. T. Abaffy, A. Malhotra, C.W. Luetje: The molecular basis for ligand specificity in a mouse olfactory receptor: A network of functionally important residues, J. Biol. Chem. 282, 1216–1224 (2007)

    Article  CAS  Google Scholar 

  44. J. Minic, M.A. Persuy, E. Godel, J. Aioun, I. Connerton, R. Salesse, E. Pajot-Augy: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening, FEBS Journal 272, 524–537 (2005)

    Article  CAS  Google Scholar 

  45. D.N. Dhanasekaran, V. Radhika, T. Proikas-Cezanne, M. Jayaraman, J. Ha: Heterologous expression of olfactory receptors for targeted chemosensing, Ann. NY Acad. Sci. 1170, 157–160 (2009)

    Article  CAS  Google Scholar 

  46. Y. Fukutani, T. Nakamura, M. Yorozu, J. Ishii, A. Kondo, M. Yohda: The N-terminal replacement of an olfactory receptor for the development of a yeast-based biomimetic odor sensor, Biotechnol. Bioeng. 109, 205–212 (2012)

    Article  CAS  Google Scholar 

  47. G. Sanz, E. Pajot-Augy: Deciphering activation of olfactory receptors using heterologous expression in Saccharomyces cerevisiae and bioluminescence resonance energy transfer, Methods Mol. Biol. 1003, 149–160 (2013)

    Article  CAS  Google Scholar 

  48. A.A. Gimelbrant, T.D. Stoss, T.M. Landers, T.S. McClintock: Truncation releases olfactory receptors from the endoplasmic reticulum of heterologous cells, J. Neurochem. 72, 2301–2311 (1999)

    Article  CAS  Google Scholar 

  49. M. Lu, F. Echeverri, B.D. Moyer: Endoplasmic reticulum retention, degradation, and aggregation of olfactory G-protein coupled receptors, Traffic. 4, 416–433 (2003)

    Article  CAS  Google Scholar 

  50. M. Lu, L. Staszewski, F. Echeverri, H. Xu, B.D. Moyer: Endoplasmic reticulum degradation impedes olfactory G-protein coupled receptor functional expression, BMC Cell Biol. 5, 520810 (2004)

    Article  CAS  Google Scholar 

  51. A. Yasuoka, Y. Emori, K. Abe: Addition of signal leader sequences to the N-termini of olfactory receptor proteins enhances their expression in Xenopus oocyte, Biosci. Biotechnol Biochem. 64, 1688–1695 (2000)

    Article  CAS  Google Scholar 

  52. I. Gaillard, S. Rouquier, J.P. Pin, P. Mollard, S. Richard, C. Barnabe, J. Demaille, D. Giorgi: A single olfactory receptor specifically binds a set of odorant molecules, Eur. J. Neurosci. 15, 409–418 (2002)

    Article  Google Scholar 

  53. B.D. Shepard, N. Natarajan, R.J. Protzko, O.W. Acres, J.L. Pluznick: A cleavable N-terminal signal peptide promotes widespread olfactory receptor surface expression in HEK293T cells, PLoS One 8, e68758 (2013)

    Article  CAS  Google Scholar 

  54. S. Pifferi, A. Menini, T. Kurahashi: Signal transduction in vertebrate olfactory cilia. In: The Neurobiology of Olfaction, ed. by A. Menini (CRC Press, Boca Raton 2010)

    Google Scholar 

  55. S. DeMaria, J. Ngai: The cell biology of smell, J. Cell Biol. 191, 443–452 (2010)

    Article  CAS  Google Scholar 

  56. R.M. Paredes, J.C. Etzler, L.T. Watts, W. Zheng, J.D. Lechleiter: Chemical calcium indicators, Methods 46, 143–151 (2008)

    Article  CAS  Google Scholar 

  57. D. Schild, D. Restrepo: Transduction mechanisms in vertebrate olfactory receptor cells, Physiol. Rev. 78, 429–466 (1998)

    CAS  Google Scholar 

  58. L.E.C. Von Dannecker, A.F. Mercadante, B. Malnic: Ric-8B promotes functional expression of odorant receptors, Proc. Natl. Acad. Sci. USA 103, 9310–9314 (2006)

    Article  CAS  Google Scholar 

  59. S. Katada, T. Nakagawa, H. Kataoka, K. Touhara: Odorant response assays for a heterologously expressed olfactory receptor, Biochem. Biophys. Res. Commun. 305, 964–969 (2003)

    Article  CAS  Google Scholar 

  60. D. Gabriel, M. Vernier, M.J. Pfeifer, B. Dasen, L. Tenaillon, R. Bouhelal: High throughput screening technologies for direct cyclic AMP measurement, Assay. Drug Dev. Technol. 1, 291–303 (2003)

    Article  CAS  Google Scholar 

  61. S.D. Liberles, L.B. Buck: A second class of chemosensory receptors in the olfactory epithelium, Nature 442, 645–650 (2006)

    Article  CAS  Google Scholar 

  62. N. Benbernou, S. Robin, S. Tacher, M. Rimbault, M. Rakotomanga, F. Galibert: cAMP and IP3 signaling pathways in HEK293 cells transfected with canine olfactory receptor genes, J. Hered. 102, S47–S61 (2011)

    Article  CAS  Google Scholar 

  63. O. Baud, S. Etter, M. Spreafico, L. Bordoli, T. Schwede, H. Vogel, H. Pick: The mouse eugenol odorant receptor: Structural and functional plasticity of a broadly tuned odorant binding pocket, Biochemistry 50, 843–853 (2011)

    Article  CAS  Google Scholar 

  64. S. Etter: Characterizing Molecular Recognition Principles of Specific Odorant Molecule Interactions with an Olfactory Receptor and a Nuclear Hormone Receptor, Ph.D. Thesis (EPFL, Lausanne 2007)

    Google Scholar 

  65. B.C. Heng, D. Aubel, M. Fussenegger: An overview of the diverse roles of G-protein coupled receptors (GPCRs) in the pathophysiology of various human diseases, Biotechnol. Adv. 31, 1676–1694 (2013)

    Article  CAS  Google Scholar 

  66. R. Zhang, X. Xie: Tools for GPCR drug discovery, Acta Pharmacol. Sin. 33, 372–384 (2012)

    Article  CAS  Google Scholar 

  67. A. Sosinsky, G. Glusman, D. Lancet: The genomic structure of human olfactory receptor genes, Genomics 70, 49–61 (2000)

    Article  CAS  Google Scholar 

  68. G. Glusman, A. Bahar, D. Sharon, Y. Pilpel, J. White, D. Lancet: The olfactory receptor gene superfamily: data mining, classification, and nomenclature, Mamm. Genome 11, 1016–1123 (2000)

    Article  CAS  Google Scholar 

  69. G. Glusman, I. Yanai, I. Rubin, D. Lancet: The complete human olfactory subgenome, Genome Res. 11, 685–702 (2001)

    Article  CAS  Google Scholar 

  70. S. Zozulya, F. Echeverri, T. Nguyen: The human olfactory receptor repertoire, Genome Biol. 2, RESEARCH0018.1 (2001)

    Article  Google Scholar 

  71. C. Crasto, M.S. Singer, G.M. Shepherd: The olfactory receptor family album, Genome Biol. 2, REVIEWS1027 (2001)

    Article  CAS  Google Scholar 

  72. H. Zhuang, H. Matsunami: Synergism of accessory factors in functional expression of mammalian odorant receptors, J. Biol. Chem. 282, 15284–15293 (2007)

    Article  CAS  Google Scholar 

  73. A. Ulloa-Aguirre, D. Stanislaus, J.A. Janovick, P.M. Conn: Structure-activity relationships of G protein-coupled receptors, Archives of Medical Research 30, 420–435 (1999)

    Article  CAS  Google Scholar 

  74. R.C. Araneda, A.D. Kini, S. Firestein: The molecular receptive range of an odorant receptor, Nat. Neurosci. 3, 1248–1255 (2000)

    Article  CAS  Google Scholar 

  75. Z. Peterlin, Y. Li, G. Sun, R. Shah, S. Firestein, K. Ryan: The importance of odorant conformation to the binding and activation of a representative olfactory receptor, Chem. Biol. 15, 1317–1327 (2008)

    Article  CAS  Google Scholar 

  76. S. Katada, T. Hirokawa, Y. Oka, M. Suwa, K. Touhara: Structural basis for a broad but selective ligand spectrum of a mouse olfactory receptor: Mapping the odorant-binding site, J. Neurosci. 25, 1806–1815 (2005)

    Article  CAS  Google Scholar 

  77. S. Katada, T. Hirokawa, K. Touhara: Exploring the odorant binding site of a G-protein-coupled olfactory receptor, Current Computer-Aided Drug Design 4, 123–131 (2008)

    Article  CAS  Google Scholar 

  78. M.S. Singer: Analysis of the molecular basis for octanal interactions in the expressed rat I7 olfactory receptor, Chem. Senses 25, 155–165 (2000)

    Article  CAS  Google Scholar 

  79. V. Jacquier, H. Pick, H. Vogel: Characterization of an extended receptive ligand repertoire of the human olfactory receptor OR17-40 comprising structurally related compounds, J. Neurochem. 97, 537–544 (2006)

    Article  CAS  Google Scholar 

  80. J. Reisert, D. Restrepo: Molecular tuning of odorant receptors and its implication for odor signal processing, Chem. Senses 34, 535–545 (2009)

    Article  CAS  Google Scholar 

  81. G. Sanz, T. Thomas-Danguin: H. el Hamdani, C. LePoupon, L. Briand, J.C. Pernollet, E. Guichard, A. Tromelin: Relationships between molecular structure and perceived odor quality of ligands for a human olfactory receptor, Chem. Senses 33, 639–653 (2008)

    Article  CAS  Google Scholar 

  82. X. Grosmaitre, S.H. Fuss, A.C. Lee, K.A. Adipietro, H. Matsunami, P. Mombaerts, M. Ma: SR1, a mouse odorant receptor with an unusually broad response profile, J. Neurosci. 29, 14545–14552 (2009)

    Article  CAS  Google Scholar 

  83. M. Spehr, K. Schwane, S. Heilmann, G. Gisselmann, T. Hummel, H. Hatt: Dual capacity of a human olfactory receptor, Curr. Biol. 14, R832–R833 (2004)

    Article  CAS  Google Scholar 

  84. J.L. Pluznick, R.J. Protzko, H. Gevorgyan, Z. Peterlin, A. Sipos, J. Han, I. Brunet, L.X. Wan, F. Rey, T. Wang, S.J. Firestein, M. Yanagisawa, J.I. Gordon, A. Eichmann, J. Peti-Peterdi, M.J. Caplan: Olfactory receptor responding to gut microbiota-derived signals plays a role in renin secretion and blood pressure regulation, Proc. Natl. Acad. Sci. USA 110, 4410–4415 (2013)

    Article  CAS  Google Scholar 

  85. M. Brodin, M. Laska, M.J. Olsson: Odor interaction between bourgeonal and its antagonist undecanal, Chem. Senses 34, 625–630 (2009)

    Article  CAS  Google Scholar 

  86. Y. Oka, A. Nakamura, H. Watanabe, K. Touhara: An odorant derivative as an antagonist for an olfactory receptor, Chem. Senses 29, 815–822 (2004)

    Article  CAS  Google Scholar 

  87. Y. Oka, M. Omura, H. Kataoka, K. Touhara: Olfactory receptor antagonism between odorants, EMBO Journal 23, 120–126 (2004)

    Article  CAS  Google Scholar 

  88. J. Liu, Y. Zhang, X. Lei, Z. Zhang: Natural selection of protein structural and functional properties: a single nucleotide polymorphism perspective, Genome Biol. 9, R69 (2008)

    Article  CAS  Google Scholar 

  89. L.T. May, K. Leach, P.M. Sexton, A. Christopoulos: Allosteric modulation of G protein-coupled receptors, Annu. Rev. Pharmacol. Toxicol. 47, 1–51 (2007)

    Article  CAS  Google Scholar 

  90. M. Rocheville, S.L. Garland: An industrial perspective on positive allosteric modulation as a means to discover safe and selective drugs, Drug Discovery Today: Technologies 7, e87–e94 (2010)

    Google Scholar 

  91. J.-P. Rocher: Recent Advances in drug discovery of GPCR allosteric modulators, Medchem News 3, 7–13 (2011)

    Google Scholar 

  92. C. Dulac, R. Axel: A novel family of genes encoding putative pheromone receptors in mammals, Cell 83, 195–206 (1995)

    Article  CAS  Google Scholar 

  93. J. Fleischer, K. Schwarzenbacher, H. Breer: Expression of trace amine-associated receptors in the Grueneberg ganglion, Chem. Senses 32, 623–631 (2007)

    Article  CAS  Google Scholar 

  94. D.M. Ferrero, J.K. Lemon, D. Fluegge, S.L. Pashkovski, W.J. Korzan, S.R. Datta, M. Spehr, M. Fendt, S.D. Liberles: Detection and avoidance of a carnivore odor by prey, Proc. Natl. Acad. Sci. USA 108, 11235–11240 (2011)

    Article  CAS  Google Scholar 

  95. Q. Li, W.J. Korzan, D.M. Ferrero, R.B. Chang, D.S. Roy, M. Buchi, J.K. Lemon, A.W. Kaur, L. Stowers, M. Fendt, S.D. Liberles: Synchronous evolution of an odor biosynthesis pathway and behavioral response, Curr. Biol. 23, 11–20 (2013)

    Article  CAS  Google Scholar 

  96. V. Carnicelli, A. Santoro, S. Sellari-Franceschini, S. Berrettini, R. Zucchi: Expression of trace amine-associated receptors in human nasal mucosa, Chemosensory Perception 3, 99–107 (2010)

    Article  Google Scholar 

  97. F. Wilkin, C. Verbeurgt, M. Philippeau, P. Chatelain: Profiling TAAR gene expression in human olfactory epithelium and functional characterization of the expressed hTAAR1 and hTAAR5, Chem. Senses 39, 114–115 (2014)

    Google Scholar 

  98. L.A. Hu, T. Zhou, J. Ahn, S. Wang, J. Zhou, Y. Hu, Q. Liu: Human and mouse trace amine-associated receptor 1 have distinct pharmacology towards endogenous monoamines and imidazoline receptor ligands, Biochem. J. 424, 39–45 (2009)

    Article  CAS  Google Scholar 

  99. I. Wallrabenstein, J. Kuklan, L. Weber, S. Zborala, M. Werner, J. Altmuller, C. Becker, A. Schmidt, H. Hatt, T. Hummel, G. Gisselmann: Human trace amine-associated receptor TAAR5 can be activated by trimethylamine, PLoS One 8, e54950 (2013)

    Article  CAS  Google Scholar 

  100. W.E. Grus, J. Zhang: Rapid turnover and species-specificity of vomeronasal pheromone receptor genes in mice and rats, Gene 340, 303–312 (2004)

    Article  CAS  Google Scholar 

  101. D. Trotier, C. Eloit, M. Wassef, G. Talmain, J.L. Bensimon, K.B. Doving, J. Ferrand: The vomeronasal cavity in adult humans, Chem. Senses 25, 369–380 (2000)

    Article  CAS  Google Scholar 

  102. M. Meredith: Human vomeronasal organ function: A critical review of best and worst cases, Chem. Senses 26, 433–445 (2001)

    Article  CAS  Google Scholar 

  103. I. Rodriguez, C.A. Greer, M.Y. Mok, P. Mombaerts: A putative pheromone receptor gene expressed in human olfactory mucosa, Nat. Genet. 26, 18–19 (2000)

    Article  CAS  Google Scholar 

  104. S. Takeda, S. Kadowaki, T. Haga, H. Takaesu, S. Mitaku: Identification of G protein-coupled receptor genes from the human genome sequence, FEBS Letters 520, 97–101 (2002)

    Article  CAS  Google Scholar 

  105. E. Shirokova, J.D. Raguse, W. Meyerhof, D. Krautwurst: The human vomeronasal type-1 receptor family–detection of volatiles and cAMP signaling in HeLa/Olf cells, FASEB Journal 22, 1416–1425 (2008)

    Article  CAS  Google Scholar 

  106. K. Corin, P. Baaske, S. Geissler, C.J. Wienken, S. Duhr, D. Braun, S. Zhang: Structure and function analyses of the purified GPCR human vomeronasal type 1 receptor 1, Sci. Rep. 1, 172 (2011)

    Article  CAS  Google Scholar 

  107. P.A. Brennan: Pheromones and Mammalian Behavior. In: The Nerobiology of Olfaction, ed. by A. Menini (CRC, Boca Raton 2010)

    Google Scholar 

  108. L.B. Vosshall, H. Amrein, P.S. Morozov, A. Rzhetsky, R. Axel: A spatial map of olfactory receptor expression in the Drosophila antenna, Cell 96(5), 725–736 (1999)

    Article  CAS  Google Scholar 

  109. P.J. Clyne, C.G. Warr, M.R. Freeman, D. Lessing, J. Kim, J.R. Carlson: A novel family of divergent seven-transmembrane proteins: Candidate odorant receptors in Drosophila, Neuron 22, 327–338 (1999)

    Article  CAS  Google Scholar 

  110. Q. Gao, A. Chess: Identification of candidate Drosophila olfactory receptors from genomic DNA sequence, Genomics 60, 31–39 (1999)

    Article  CAS  Google Scholar 

  111. R. Benton, S. Sachse, S.W. Michnick, L.B. Vosshall: Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo, PLoS Biology 4, e20 (2006)

    Article  CAS  Google Scholar 

  112. K. Sato, M. Pellegrino, T. Nakagawa, T. Nakagawa, L.B. Vosshall, K. Touhara: Insect olfactory receptors are heteromeric ligand-gated ion channels, Nature 452, 1002–1006 (2008)

    Article  CAS  Google Scholar 

  113. D. Wicher, R. Schafer, R. Bauernfeind, M.C. Stensmyr, R. Heller, S.H. Heinemann, B.S. Hansson: Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channels, Nature 452, 1007–1011 (2008)

    Article  CAS  Google Scholar 

  114. C.H. Wetzel, H.J. Behrendt, G. Gisselmann, K.F. Stortkuhl, B. Hovemann, H. Hatt: Functional expression and characterization of a Drosophila odorant receptor in a heterologous cell system, Proc. Natl. Acad. Sci. USA 98, 9377–9380 (2001)

    Article  CAS  Google Scholar 

  115. T. Lu, Y.T. Qiu, G. Wang, J.Y. Kwon, M. Rutzler, H.W. Kwon, R.J. Pitts, J.J. van Loon, W. Takken, J.R. Carlson, L.J. Zwiebel: Odor coding in the maxillary palp of the malaria vector mosquito Anopheles gambiae, Curr. Biol. 17, 1533–1544 (2007)

    Article  CAS  Google Scholar 

  116. G. Wang, A.F. Carey, J.R. Carlson, L.J. Zwiebel: Molecular basis of odor coding in the malaria vector mosquito Anopheles gambiae, Proc. Natl. Acad. Sci. USA 107, 4418–4423 (2010)

    Article  CAS  Google Scholar 

  117. C.W. Luetje, A.S. Nichols, A. Castro, B.L. Sherman: Functional assay of mammalian and insect olfactory receptors using Xenopus oocytes, Methods Mol. Biol. 1003, 187–202 (2013)

    Article  CAS  Google Scholar 

  118. P.L. Jones, G.M. Pask, D.C. Rinker, L.J. Zwiebel: Functional agonism of insect odorant receptor ion channels, Proc. Natl. Acad. Sci. USA 108, 8821–8825 (2011)

    Article  CAS  Google Scholar 

  119. J. Fan, F. Francis, Y. Liu, J.L. Chen, D.F. Cheng: An overview of odorant-binding protein functions in insect peripheral olfactory reception, Genet. Mol. Res. 10, 3056–3069 (2011)

    Article  CAS  Google Scholar 

  120. W.S. Leal: Odorant reception in insects: Roles of receptors, binding proteins, and degrading enzymes, Annu. Rev. Entomol. 58, 373–391 (2013)

    Article  CAS  Google Scholar 

  121. L. Briand, C. Nespoulous, J.-C. Huet, M. Takahashi, J.-C. Pernollet: Ligand binding and physico-chemical properties of ASP2, a recombinant odorant-binding protein from honeybee (Apis mellifera L), Eur. J. Biochem. 268, 752–760 (2001)

    Article  CAS  Google Scholar 

  122. A.F. Carey, J.R. Carlson: Insect olfaction from model systems to disease control, Proc. Natl. Acad. Sci. USA 108, 12987–12995 (2011)

    Article  CAS  Google Scholar 

  123. C. Flegel, S. Manteniotis, S. Osthold, H. Hatt, G. Gisselmann: Expression profile of ectopic olfactory receptors determined by deep sequencing, PLoS One 8, e55368 (2013)

    Article  CAS  Google Scholar 

  124. N. Kang, J. Koo: Olfactory receptors in non-chemosensory tissues, BMB Report 45, 612–622 (2012)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the Brussels Institute for Research and Innovation (InnovIRIS).

Author information

Authors and Affiliations

  1. ChemCom SA, Route de Lennik 802, 1070, Brussels, Belgium

    Alex Veithen, Magali Philippeau & Pierre Chatelain

Authors
  1. Alex Veithen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Magali Philippeau
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pierre Chatelain
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Alex Veithen or Pierre Chatelain .

Editor information

Editors and Affiliations

  1. Fraunhofer-Institut for Process Engineering and Packaging, Giggenhauser Str. 35, 85354, Freising, Germany

    Andrea Buettner

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Veithen, A., Philippeau, M., Chatelain, P. (2017). High Throughput Receptor Screening Assays. In: Buettner, A. (eds) Springer Handbook of Odor. Springer Handbooks. Springer, Cham. https://doi.org/10.1007/978-3-319-26932-0_22

Download citation

Publish with us

Policies and ethics

Search

Navigation