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Detection of S-Phase of Cell Division Cycle in Plant Cells and Tissues by Using 5-Ethynyl-2′-Deoxyuridine (EdU)

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Plant Microtechniques and Protocols

Abstract

A detailed analysis of the plant cell division cycle is required to understand the changes in metabolic, developmental, and physiological processes. Determination of DNA synthesis phase (S-phase) of the cell division cycle is particularly important to assess the proliferative status of a given cell, tissue, or organ. Several methods exist to detect and quantify the S-phase of the cell division cycle. In comparison to frequently used detection by bromodeoxyuridine (BrdU) and tritiated thymidine incorporation, recently introduced ethynyl deoxyuridine method (EdU) described here, affords several advantages. EdU assay offers a simple, rapid, and nonradioactive method for proliferation analysis in plant cells while preserving morphological integrity. Here, we describe the 5-ethynyl-2′-deoxyuridine (EdU)-based S-phase assay for use in plant cells and tissues.

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Author information

Authors and Affiliations

  1. Cellular Imaging Laboratory, Biological Research Centre, Hungarian Academy of Sciences, Temesvári körút 62, 6726, Szeged, Hungary

    Soujanya Kuntam & Ferhan Ayaydin

Authors
  1. Soujanya Kuntam
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  2. Ferhan Ayaydin
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Correspondence to Ferhan Ayaydin .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, University of Calgary, CALGARY, Alberta, Canada

    Edward Chee Tak Yeung

  2. Department of Plant Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Claudio Stasolla

  3. Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Michael John Sumner

  4. Center for Basic Research in Digestive Disease, Mayo Clinic , Rochester, Minnesota, USA

    Bing Quan Huang

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Kuntam, S., Ayaydin, F. (2015). Detection of S-Phase of Cell Division Cycle in Plant Cells and Tissues by Using 5-Ethynyl-2′-Deoxyuridine (EdU). In: Yeung, E., Stasolla, C., Sumner, M., Huang, B. (eds) Plant Microtechniques and Protocols. Springer, Cham. https://doi.org/10.1007/978-3-319-19944-3_18

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