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Hybrid Sol/Gels for DNA Arrays and Other Lab-on-a-Chip Applications

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Handbook of Sol-Gel Science and Technology

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Abstract

This work describes various ways to improve the attachment, retention, and functionality of DNA strands on and within sol/gel coatings deposited on transparent glass slides for lab-on-a-chip applications. The DNA strands were tagged with fluorescent molecules to quantify their retention using confocal scanning fluorescence microscopy. In addition to conventional silicate gels, hybrid gels containing aminosilane were also studied. Here, 50–60 nm thick, large-pore gel coatings were used to immobilize DNA within the bulk pore-space of the gel, in addition to spotting the surface with DNA strands. Some advantages of using aminosilane include improved adsorption of the DNA, control of bulk pore-size and especially the ability to increase the spot density due to improved confinement of the spots. This lead naturally to micropatterning of DNA arrays using sol/gels for soft lithography. Although new methods for assaying DNA strands, as well as improvements in soft lithography with sol/gels, have been developed since this work was first published, the design concepts and processing methods are still relevant.

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Acknowledgment

The authors gratefully acknowledge the Center for Nanoscale Science, an NSF Materials Research and Engineering Center (MRSEC) at the Pennsylvania State University and also Schott Glass Technologies (Duryea, PA, USA) for their support.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, Materials Research Institute, The Pennsylvania State University, University Park, PA, 16802, USA

    Carlo G. Pantano

  2. Department of Metallurgical and Materials Engineering, Middle East Technical University, Ankara, Turkey

    Caner Durucan

Authors
  1. Caner Durucan
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  2. Carlo G. Pantano
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Correspondence to Carlo G. Pantano .

Editor information

Editors and Affiliations

  1. Rutgers University, Piscataway, New Jersey, USA

    Lisa Klein

  2. Madrid, Spain

    Mario Aparicio

  3. City University of New York, New York, USA

    Andrei Jitianu

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Durucan, C., Pantano, C.G. (2016). Hybrid Sol/Gels for DNA Arrays and Other Lab-on-a-Chip Applications. In: Klein, L., Aparicio, M., Jitianu, A. (eds) Handbook of Sol-Gel Science and Technology. Springer, Cham. https://doi.org/10.1007/978-3-319-19454-7_77-1

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  • DOI: https://doi.org/10.1007/978-3-319-19454-7_77-1

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  • Publisher Name: Springer, Cham

  • Online ISBN: 978-3-319-19454-7

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