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Using Next-Generation Sequencing to Reveal Patterns of Chromosomal Alterations in Oral Verrucous Lesions

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Next Generation Sequencing in Cancer Research, Volume 2

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Abstract

Oral verrucous carcinoma is considered to be a histological subtype of oral squamous cell carcinoma, which generally follows a more indolent clinical course. It presents a specific clinical challenge in that it can be difficult to reach a definitive diagnosis and harbours the potential to transform into the more aggressive squamous cell carcinoma. In this chapter, we will discuss the background of this disease and the potential and previous role of next-generation sequencing (NGS) in head and neck cancer. The use of low coverage NGS to produce copy number variation (CNV) data and demonstrate how different computational methods can be applied to that data to analyse patterns and identify targets of interest. The application of NGS to detect and determine the prevalence of human papillomavirus in this disease will also be discussed.

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Author information

Authors and Affiliations

  1. Leeds Institute of Cancer and Pathology, St James’ University Hospital, Wellcome Trust Brenner Building, Leeds, LS9 7TF, UK

    Manar Samman M.Sc. & Neeraj Sethi M.B.Ch.B., M.R.C.S., D.O.H.N.S.

  2. King Fahad Medical City, Riyadh, Saudi Arabia

    Manar Samman M.Sc.

Authors
  1. Manar Samman M.Sc.
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  2. Neeraj Sethi M.B.Ch.B., M.R.C.S., D.O.H.N.S.
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Correspondence to Manar Samman M.Sc. .

Editor information

Editors and Affiliations

  1. Department of Pathology and Laboratory, Medicine, Southern Alberta Cancer Research Institute, University of Calgary, Calgary, Alberta, Canada

    Wei Wu

  2. Faculty of Science, Biochemistry Department, Center of Innovation in Personalized Medicine, King Fahd Center for Medical Research, King Abdulaziz University, Jeddah, Saudi Arabia

    Hani Choudhry

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Samman, M., Sethi, N. (2015). Using Next-Generation Sequencing to Reveal Patterns of Chromosomal Alterations in Oral Verrucous Lesions. In: Wu, W., Choudhry, H. (eds) Next Generation Sequencing in Cancer Research, Volume 2. Springer, Cham. https://doi.org/10.1007/978-3-319-15811-2_19

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