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Handbook for Azospirillum pp 287–305Cite as

Methods to Study 1-Aminocyclopropane-1-carboxylate (ACC) Deaminase in Plant Growth-Promoting Bacteria

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Abstract

The lowering of plant ethylene levels by the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase is one of the key mechanisms employed by plant growth-promoting bacteria (PGPB) to facilitate plant growth. Since its discovery, it has been detected in both fungi and bacteria. It has been shown by a large number of workers in a wide range of bacteria that the activity of this enzyme in PGPB is important during normal plant development and also protects plants from the deleterious effects of a wide range of environmental stresses. ACC deaminase-containing PGPB bound to a plant act as a sink for ACC, thereby lowering ethylene levels in plant tissues. The result of the functioning of this enzyme is an increase in the growth of plant roots and shoots and a reduction of the inhibitory effects of ethylene synthesis especially during stressful conditions. This chapter briefly summarizes the current knowledge of various ACC deaminases emphasizing the use of ACC deaminase-containing bacteria in promoting plant growth under diverse biotic and abiotic stresses, and describes methods for the isolation and study of ACC deaminase-containing bacteria.

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Authors and Affiliations

  1. ICAAM—Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Pólo da Mitra, Ap. 94, Évora, 7002-554, Portugal

    Clarisse Brígido

  2. Department of Biology, University of Waterloo, Waterloo, ON, Canada, N21 3G1

    Jin Duan & Bernard R. Glick

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  1. Clarisse Brígido
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  2. Jin Duan
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  3. Bernard R. Glick
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Correspondence to Clarisse Brígido .

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  1. Laboratorio de Fisiología Vegetal y de la Interacción Planta-Microorganismo, Universidad Nacional de Rio Cuarto, Rio Cuarto, Córdoba, Argentina

    Fabricio Dario Cassán

  2. Faculty of Agricultue Food and Environme, The Hebrew University of Jerusalem, Rehovot, Central, Israel

    Yaacov Okon

  3. Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Balcarce, Argentina

    Cecilia M. Creus

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Brígido, C., Duan, J., Glick, B.R. (2015). Methods to Study 1-Aminocyclopropane-1-carboxylate (ACC) Deaminase in Plant Growth-Promoting Bacteria. In: Cassán, F., Okon, Y., Creus, C. (eds) Handbook for Azospirillum. Springer, Cham. https://doi.org/10.1007/978-3-319-06542-7_16

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