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Birkhäuser

Methods in Non-Aqueous Enzymology

  • Book
  • © 2000

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Editors:
  1. Munishwar Nath Gupta

    1. Chemistry Department, Indian Institute of Technology, Delhi Hauz Khas, New Delhi, India

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Part of the book series: Methods and Tools in Biosciences and Medicine (MTBM)

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Table of contents (12 chapters)

  1. Front Matter

    Pages I-X
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  2. Non-Aqueous Enzymology: Issues and Persspectives

    • Munishwar N. Gupta
    Pages 1-13

  3. Importance of Water Activity for Enzyme Catalysis in Non-Aqueous Organic Systems

    • Thorleif Anthonsen, Birte J. Sjursnes
    Pages 14-35

  4. Engineering of Enzymes via Immobili-zation and Post-Immobilization Techniques: Preparation of Enzyme Derivatives with Improved Stability in Organic Media

    • Gloria Fernández-Lorente, Roberto Fernández-Lafuente, Pilar ArmisĂ©n, Pilar Sabuquillo, Cesar Mateo, JosĂ© M. Guisán
    Pages 36-51

  5. Immobilization of Lipases for Use in Non-Aqueous Reaction Systems

    • John A. Bosley, Alan D. Peilow
    Pages 52-69

  6. Applications of Enzymes and Membrane Technology in Fat and Oil Processing Processing

    • Mitsutoshi Nakajima, Jonathan B. Snape, Sunil Kumar Khare
    Pages 70-89

  7. Strategies for Improving the Lipase-Catalyzed Preparation of Chiral Compounds

    • Uwe T. Bornscheuer
    Pages 90-109

  8. Peptide Synthesis in Non-Aqueous Media

    • Pere ClapĂ©s, Gloria Caminal, Josep A. Feliu, Josep LĂłpez-SantĂ­n
    Pages 110-132

  9. Enzyme Selectivity in Organic Media

    • Gianluca Ottolina, Sergio Riva
    Pages 133-145

  10. Sugar Transformations Using Enzymes in Non-Aqueous Media

    • Sergio Riva, Gabriella Roda
    Pages 146-159

  11. Reversed Micelles as Microreactors: N-terminal Acylation of RNase A and its Characterization

    • JoĂ«l Chopineau, Bernard Lagoutte, Daniel Thomas, Dominique Domurado
    Pages 160-173

  12. Analysis in Non-Aqueous Milieu Using Thermistors

    • Kumaran Ramanathan, Birgitta Rees Jönsson, Bengt Danielsson
    Pages 174-194

  13. Importance of the Medium for in vitro and in vivo Protein Folding Mechanisms: Biomedical Implications

    • Munishwar Nath Gupta
    Pages 195-211

  14. Back Matter

    Pages 212-218
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Keywords

About this book

Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffers) is that the awareness of different parameters that control and influence the behaviour of enzymes in such environments has emerged rather slowly. Science is about being able to repeat what somebody else has done. Absence of knowledge about such well-defined parameters/fac­ tors has sometimes made some workers rather cautious and diffident about using this approach in their laboratories. But for this, non-aqueous enzymol­ ogy would be more widely practised. It is these thoughts that made me feel that the availability of some well-defined protocols for various applications invol­ ving enzymes in non-aqueous environments would further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac­ tivity and stabilization via immobilization. Some subsequent chapters provide the protocols for transformations involving lipids and carbohydrates, peptide synthesis, and preparation of chiral compounds. The disproportionate focus on lipases is not a coincidence; this class of enzymes has been used more often than others in non-aqueous enzymology.

Editors and Affiliations

  • Chemistry Department, Indian Institute of Technology, Delhi Hauz Khas, New Delhi, India

    Munishwar Nath Gupta

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