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Proteomics in Functional Genomics pp 1–27Cite as

Birkhäuser

Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis

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Part of the book series: EXS ((EXS,volume 88))

Summary

The high resolving power of two-dimensional polyacrylamide gel electrophoresis 2D-PAGE and its full analytical and preparative potential have been described with special emphasis on reproducibility and standardization of protein spot patterns, enhanced protein detection sensitivity, and computer analysis database development. New methodologies for peptide mass fingerprinting, peptide, sequence, and fragmention tagging have been highlighted. Major challenges associated with 2D-PAGE/mass spectrometric protein sequencing were outlined which need to be addressed in the future, including sample enrichment, use of alternative gel matrices, improvements in separation systems interfaced directly to the mass spectrometer, and design of high-sensitivity instruments with very high mass ranges. It is hoped that comparative studies to identify, quantitate, and characterize proteins differentially expressed in normal versus diseased cells would give insight into mechanisms of pathogenesis and allow the development of a way to control both the etiology and the course of diseases.

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Authors and Affiliations

  1. Laboratory of Cell Biology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Bethesda, MD, 20892, USA

    Ettore Appella & Kazuyasu Sakaguchi

  2. Laboratory of Experimental Carcinogenesis, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, 37 Convent Drive, Bethesda, MD, 20892, USA

    David Arnott

  3. Protein Chemistry Department, Genentech, Inc, 1 DNA Way, South San Francisco, CA, 94080, USA

    Peter J. Wirth

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  1. Ettore Appella
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  2. David Arnott
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  3. Kazuyasu Sakaguchi
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  4. Peter J. Wirth
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Editors and Affiliations

  1. Laboratoire de Chimie des Substances Naturelles URA C.N.R.S. No. 401, Muséum National d’Histoire Naturelle, 63, rue Buffon, F-75005, Paris, France

    P. Jollès

  2. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77, Stockholm, Sweden

    H. Jörnvall

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Appella, E., Arnott, D., Sakaguchi, K., Wirth, P.J. (2000). Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis. In: Jollès, P., Jörnvall, H. (eds) Proteomics in Functional Genomics. EXS, vol 88. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8458-7_1

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  • DOI: https://doi.org/10.1007/978-3-0348-8458-7_1

  • Publisher Name: Birkhäuser, Basel

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