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Isolation and Regulation of Expression of the Neurospora Crassa Copper Metallothionein Gene

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Metallothionein II

Part of the book series: Experientia Supplementum ((EXS,volume 52))

Abstract

The N. crassa CuMT gene has been cloned and its nucleotide sequence determined., To this end an MT specific undecanucleotide was synthesized and used for cDNA synthesis with enriched MT mRNA as a template. Sequence analysis of the cDNA obtained allowed the synthesis of a unique 21mer which was used as a hybridization probe to screen a genomic DNA library of N. crassa. Several positive clones were isolated and subjected to restriction and sequence analysis. In agreement with the published amino acid sequence, the gene codes for a polypeptide of 26 amino acid residues in length. The coding region is interrupted by a small intron. Compared to the structure of mammalian MT genes the intron-exon boundaries are located in different sequence positions. The induction of MT mRNA was studied by Northern analysis. Maximum levels of MT mRNA were detected about 1 hour after addition of copper ions to mycelium of N. crassa. The half-life time of the messenger was estimated as 2.5 hours. The CuMT amounts reach a maximum level at 3 hours after induction and thereafter remain constant.

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Author information

Authors and Affiliations

  1. Biochemisches Institut Universität Zürich, CH-8057, Zürich, Switzerland

    K. Münger, U. A. Germann & Dr. Konrad Lerch

  2. Biochemisches Institut der Universität Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland

    Dr. Konrad Lerch

Authors
  1. K. Münger
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  2. U. A. Germann
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  3. Dr. Konrad Lerch
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Editor information

Editors and Affiliations

  1. Institute of Biochemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland

    Jeremias H. R. Kägi

  2. Dept. of Environmental Medicine, Hokkaido University, Kita-ku, Sapporo, 060, Japan

    Yutaka Kojima

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© 1987 Springer Basel AG

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Münger, K., Germann, U.A., Lerch, K. (1987). Isolation and Regulation of Expression of the Neurospora Crassa Copper Metallothionein Gene. In: Kägi, J.H.R., Kojima, Y. (eds) Metallothionein II. Experientia Supplementum, vol 52. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6784-9_37

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  • DOI: https://doi.org/10.1007/978-3-0348-6784-9_37

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-6786-3

  • Online ISBN: 978-3-0348-6784-9

  • eBook Packages: Springer Book Archive

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