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Purification of In Vivo or In Vitro-Assembled RNA-Protein Complexes by RNA Centric Methods

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Advanced Technologies for Protein Complex Production and Characterization

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 3234))

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Abstract

Throughout their entire life cycle, RNAs are associated with RNA-binding proteins (RBPs), forming ribonucleoprotein (RNP) complexes with highly dynamic compositions and very diverse functions in RNA metabolism, including splicing, translational regulation, ribosome assembly. Many RNPs remain poorly characterized due to the challenges inherent in their purification and subsequent biochemical characterization. Therefore, developing methods to isolate specific RNA-protein complexes is an important initial step toward understanding their function. Many elegant methodologies have been developed to isolate RNPs. This chapter describes different approaches and methods devised for RNA-specific purification of a target RNP. We focused on general methods for selecting RNPs that target a given RNA under conditions favourable for the copurification of associated factors including RNAs and protein components of the RNP.

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Acknowledgments

The authors are supported by institutional funds from the Centre National de la Recherche Scientifique (CNRS), the Université de Strasbourg, and grant ANR-17-CE11-0024-01 and ANR-17-CE12-0025-01.

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Authors and Affiliations

  1. Architecture et Réactivité de l’ARN, Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, Strasbourg, France

    Aurélie Janvier, Hassan Hayek, Fatima Alghoul, Lauriane Gross, Christine Allmang, Franck Martin & Gilbert Eriani

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  1. Aurélie Janvier
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  2. Hassan Hayek
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  3. Fatima Alghoul
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  4. Lauriane Gross
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  5. Christine Allmang
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  6. Franck Martin
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  7. Gilbert Eriani
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Correspondence to Gilbert Eriani .

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Editors and Affiliations

  1. Centro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), Madrid, Spain

    M. Cristina Vega

  2. Abvance Biotech SL, Madrid, Spain

    Francisco J. Fernández

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Janvier, A. et al. (2024). Purification of In Vivo or In Vitro-Assembled RNA-Protein Complexes by RNA Centric Methods. In: Vega, M.C., Fernández, F.J. (eds) Advanced Technologies for Protein Complex Production and Characterization. Advances in Experimental Medicine and Biology, vol 3234. Springer, Cham. https://doi.org/10.1007/978-3-031-52193-5_2

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