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Circulating Tumour Cell Isolation and Molecular Profiling; Potential Therapeutic Intervention

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Circulating Tumor Cells

Part of the book series: Current Cancer Research ((CUCR))

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Abstract

Comprehensive tumour characterisation is indispensable for patients to receive targeted therapy. The use of liquid biopsy, particularly circulating tumour cells (CTC), has shown great promise in the treatment and management of cancer patients. An in-depth understanding of CTCs at the cellular and molecular level can provide clues as to the mechanisms of cancer dissemination and the pathways responsible for conferring intrinsic and acquired resistance to therapeutic agents. Herein, we discuss the current methods of CTC isolation and analysis at the single-cell resolution for therapeutic applications in the management of cancer.

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Acknowledgements

M. E. W. would like to acknowledge the support of the Australian Research Council through Discovery Project Grants (DP170103704 and DP180103003) and the National Health and Medical Research Council through the Career Development Fellowship (APP1143377). JPT would like to acknowledged the financial support of Guangzhou Laboratory.

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Author notes

  1. Payar Radfar and Hamidreza Aboulkheyr Es contributed equally with all other contributors.

Authors and Affiliations

  1. School of Biomedical Engineering, University of Technology Sydney, Sydney, Australia

    Payar Radfar, Hamidreza Aboulkheyr Es & Majid Ebrahimi Warkiani

  2. School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, Australia

    Arutha Kulasinghe

  3. Translational Research Institute, Brisbane, Australia

    Arutha Kulasinghe

  4. Institute of Molecular Cell Biology A*STAR, Singapore, Singapore

    Jean Paul Thiery

  5. Guangzhou Laboratory, Guangzhou, China

    Jean Paul Thiery

  6. Institute of Molecular Medicine, Sechenov First Moscow State University, Moscow, Russia

    Jean Paul Thiery & Majid Ebrahimi Warkiani

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  1. Payar Radfar
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  2. Hamidreza Aboulkheyr Es
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  3. Arutha Kulasinghe
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Correspondence to Jean Paul Thiery .

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  1. Department of Pathology and Immunology, Washington University in St. Louis School of Medicine, St. Louis, MO, USA

    Richard J. Cote

  2. ACTC Lab, Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Athens, Greece

    Evi Lianidou

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Radfar, P., Es, H.A., Kulasinghe, A., Thiery, J.P., Warkiani, M.E. (2023). Circulating Tumour Cell Isolation and Molecular Profiling; Potential Therapeutic Intervention. In: Cote, R.J., Lianidou, E. (eds) Circulating Tumor Cells. Current Cancer Research. Springer, Cham. https://doi.org/10.1007/978-3-031-22903-9_14

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