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Photodynamic Inactivation in Vitro of the Pathogenic Fungus Paracoccidioides brasiliensis

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XXVII Brazilian Congress on Biomedical Engineering (CBEB 2020)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 83))

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Abstract

Paracoccidioidomycosis (PCM) is a disease caused by the fungus Paracoccidioides brasiliensis (Pb), manifesting as a systemic mycosis, which can lead to death. This study evaluated the action of in vitro Photodynamic Inativation (PDI) on Pb yeast cells through the LED light source (LEC Prime WL—MMOptics: 455 nm, 0.8 W/cm2 and 0.2 W with 6 mm tip) associated with the photosensitizer (Fs) curcumin 98%. The photodynamic action was performed with 4 groups and 6 application cycles of the technique, fractionated every 5 min, for a total cycle of 30 min. The tests were performed in quintuplicate and the groups were divided into: group 1—without Fs in its composition and without LED irradiation (L−F−); group 2—without Fs and with exposure to irradiation (L+F−); group 3—with Fs and without exposure to LED irradiation (L−F+); and group 4—composed with Fs and exposed to LED irradiation (L+F+). The results showed that the reduction of Pb, measured by the means of CFU Log, was significantly higher in group 4 (0.7748 ± 0.1876) when related to group 1 (2.399 ± 0.09470) (p < 0.001), group 2 (2.178 ± 0.08214) (p < 0.001) and group 3 (1.818 ± 0.09987) (p < 0.001). There was elimination of the fungus P. brasiliensis from the 20th minute of irradiation in the group exposed to the LED associated to the presence of Fs (p < 0.001). We concluded that PDI associated with the administration of curcumin 98% is capable of promoting the in vitro elimination of the pathogenic fungus Paracoccidioides brasiliensis.

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Acknowledgements

Thank the Universidade Brasil University for the supporting during Master Post Graduation Course, Anhembi Morumbi University and the Coordination for the Improvement of Higher Education Personnel (CAPES) for financing this research.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Anhembi Morumbi University, Doutor Altino Bondensan Road, 500, São José dos Campos Technology Park - Eugênio de Mello, São José dos Campos, São Paulo, Brazil

    José Alexandre da Silva Júnior & A. U. Fernandes

  2. Bioengineering and Biomedical Engineering Graduate Program, University Brazil, São Paulo, Brazil

    R. S. Navarro, D. I. Kozusny-Andreani & L. S. Feitosa

Authors
  1. José Alexandre da Silva Júnior
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  2. R. S. Navarro
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  3. A. U. Fernandes
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  4. D. I. Kozusny-Andreani
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  5. L. S. Feitosa
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Editors and Affiliations

  1. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Teodiano Freire Bastos-Filho

  2. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Eliete Maria de Oliveira Caldeira

  3. Department of Electrical Engineering, Universidade Federal do Espírito Santo, Vitória, Brazil

    Anselmo Frizera-Neto

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da Silva Júnior, J.A., Navarro, R.S., Fernandes, A.U., Kozusny-Andreani, D.I., Feitosa, L.S. (2022). Photodynamic Inactivation in Vitro of the Pathogenic Fungus Paracoccidioides brasiliensis. In: Bastos-Filho, T.F., de Oliveira Caldeira, E.M., Frizera-Neto, A. (eds) XXVII Brazilian Congress on Biomedical Engineering. CBEB 2020. IFMBE Proceedings, vol 83. Springer, Cham. https://doi.org/10.1007/978-3-030-70601-2_175

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  • DOI: https://doi.org/10.1007/978-3-030-70601-2_175

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