Abstract
The gene expression of Prox-1 and Hif-1a for the isolated primo vessels (PVs) and composite lymphatic vessels (LVs) containing PVs (LVs + PVs) was investigated by RNA-sequencing (Seq) and quantitative polymerase chain reaction (qRT-PCR) analysis. RNA-Seq on the passed 10 samples on RNA-QC for two experimental groups with PVs and PVs + LVs proceeded to the library construction stage automatically and analyzed differentially expressed genes (DEGs). From the real-time qRT-PCR analysis data, we found the marker genes of Prox-1 and Hif-1a were enriched and decreased in an isolated PVs compared to LVs, respectively. Based on mRNA transcriptional data, Prox-1 and Hif-1a were increased and decreased in PVs compared to LVs + PVs under lipopolysaccharide (LPS) treatment and relieved by acupuncture electric stimulation (AES), respectively. This finding indicates that high and low levels of Prox-1 and Hif-1a may be involved in the function of PVs and that pathophysiological and physiological condition could progress into inflamed lymphatic endothelial cells expanding the PV within the LV.
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Lee BS, Lee BC, Park JE et al (2014) Primo vascular system in human umbilical cord and placenta. J Acupunct Meridian Stud 7:291–297
Bae KH, Kwon HM, Soh KS (2015) DNA budding of primo microcells. J Acupunct Meridian Stud 8:103–104
Soh KS (2009) Bonghan circulatory system as an extension of acupuncture meridians. J Acupunct Meridian Stud 2:93–106
Noh YI, Rho M, Yoo YM et al (2012) Isolation and morphological features of primo vessels in rabbit lymph vessels. J Acupunct Meridian Stud 5:201–205
Wigle JT, Oliver G (1999) Prox1 function is required for the development of the murine lymphatic system. Cell 98:769–778
Tsuchida S, Arai Y, Takahashi KA et al (2014) HIF-1α-induced HSP70 regulates anabolic responses in articular chondrocytes under hypoxic conditions. J Orthop Res 32:975–980
Lee HR, Rho M, Hong YJ et al (2015) Primo vessel stressed by lipopolysaccharide in rabbits. J Acupunct Meridian Stud 8:301–306
Shin JY, Ji JO, Choi DW et al (2019) Expression of genes in primo vasculature floating in lymphatic endothelium under lipopolysaccharide and acupuncture electric stimulation. J Acupunct Meridian Stud 12:3–10
Kataru RP, Kim H, Jang C, Choi DK, Koh BI, Kim M et al (2011) T lymphocytes negatively regulate lymph node lymphatic vessel formation. Immunity 34:96–107
Acknowledgments
This work was supported by the National Research Foundation of Korea (NRF) funded by the Korea government (Ministry of Science and ICT) under Grant No. 2016R1E1A2A01953467.
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Shin, JY., Choi, JG., Shin, S., Yeo, S., Lee, SS. (2021). Gene Expression of Prox-1 and Hif-1a in Primo Vessels Inside Lymph Vessels of the Rabbit. In: Nemoto, E.M., Harrison, E.M., Pias, S.C., Bragin, D.E., Harrison, D.K., LaManna, J.C. (eds) Oxygen Transport to Tissue XLII. Advances in Experimental Medicine and Biology, vol 1269. Springer, Cham. https://doi.org/10.1007/978-3-030-48238-1_61
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DOI: https://doi.org/10.1007/978-3-030-48238-1_61
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