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MARCO Gene Variations and Their Association with Cardiovascular Diseases Development: An In-Silico Analysis

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Bioinformatics and Biomedical Engineering (IWBBIO 2020)

Part of the book series: Lecture Notes in Computer Science ((LNBI,volume 12108))

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Abstract

Cardiovascular diseases (CVDs) represent the leading cause of morbidity and mortality in both developed and developing countries. They have complex etiology, influenced by several risk factors including the genetic component. The genetic variations were shown to be highly associated with different CVD forms, in this objective we proceeded to analyze the Macrophage Receptor with Collagen structure gene (MARCO), we performed an in-silico study with a genomic functional analysis, to evaluate the mutations’ effects on the proteins’ structures and functionalities. Indeed, we used dbSNP to retrieve single nucleotide polymorphisms (SNPs) of MARCO gene. We proceeded then to a filtration and a stability analysis using several bioinformatics tools to evaluate the most deleterious variations. Moreover we predicted the 3D structures of the encoded proteins by MARCO gene, which was validated using PROCHECK. Then we analyzed and visualize the proteins’ 3D structures.

The extraction of the human MARCO gene SNPs revealed that dbSNP contains more than 14000 SNPs. The filtration process revealed the variations G241V and G262W to be the most deleterious SNPs, indeed, I-Mutant and DUET showed decreased protein stability. The validation using PROCHECK revealed a total of 89.9% MARCO protein residues to be in the favored region.

As conclusion, our results let suggesting that G241V and G262W variations can cause alteration in the proteins’ structures and functions. Hence, to improve the health management, screening precariously these variants, can be useful as model for CVD diagnosis and helpful in pharmacogenomics.

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Acknowledgement

We acknowledge the h3ABioNet network (African Bioinformatics Network for H3Africa) for the scientific support.

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Authors and Affiliations

  1. Chlamydiae and Mycoplasma Laboratory, Institut Pasteur du Maroc, 20360, Casablanca, Morocco

    Kholoud Sanak, Maryame Azzouzi & Fouzia Radouani

  2. IRDA Team, ENSIAS, Mohammed V University, 10112, Rabat, Morocco

    Kholoud Sanak & Mounia Abik

  3. Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Faculté des sciences Aîn-Chock, 20100, Casablanca, Morocco

    Maryame Azzouzi

Authors
  1. Kholoud Sanak
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  2. Maryame Azzouzi
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  4. Fouzia Radouani
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Corresponding author

Correspondence to Fouzia Radouani .

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Editors and Affiliations

  1. University of Granada, Granada, Spain

    Ignacio Rojas

  2. University of Granada, Granada, Spain

    Olga Valenzuela

  3. University of Granada, Granada, Spain

    Fernando Rojas

  4. University of Granada, Granada, Spain

    Luis Javier Herrera

  5. University of Chicago and Fundacion Progreso y Salud, Granada, Spain

    Francisco Ortuño

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Sanak, K., Azzouzi, M., Abik, M., Radouani, F. (2020). MARCO Gene Variations and Their Association with Cardiovascular Diseases Development: An In-Silico Analysis. In: Rojas, I., Valenzuela, O., Rojas, F., Herrera, L., Ortuño, F. (eds) Bioinformatics and Biomedical Engineering. IWBBIO 2020. Lecture Notes in Computer Science(), vol 12108. Springer, Cham. https://doi.org/10.1007/978-3-030-45385-5_19

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  • DOI: https://doi.org/10.1007/978-3-030-45385-5_19

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-45384-8

  • Online ISBN: 978-3-030-45385-5

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