Abstract
The Ras-related GTPase Rap has been implicated in multiple cellular functions. In particular, Rap1 is a crucial regulator of both inside-out integrin activation and outside-in E-cadherin-mediated signaling. Thus, Rap1 was proposed as a fundamental regulator of the cross talk between cadherins and integrins. We provide microscopic techniques to study subcellular localization of Rap1 protein in the crosstalk between integrins and cadherins.
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References
Kitayama H, Sugimoto Y, Matsuzaki T et al (1989) A ras-related gene with transformation suppressor activity. Cell 56:77–84
Bourne HR, Sanders DA, McCormick F (1990) The GTPase superfamily: a conserved switch for diverse cell functions. Nature 348:125–132
Jeyaraj SC, Unger NT, Chotani MA (2011) Rap1 GTPases: an emerging role in the cardiovasculature. Life Sci 88:645–652
Bos JL, de Rooij J, Reedquist KA (2001) Rap1 signalling: adhering to new models. Nat Rev Mol Cell Biol 2:369–377
Caron E (2003) Cellular functions of the rap1 GTP-binding protein: a pattern emerges. J Cell Sci 116:435–440
Gloerich M, Bos JL (2011) Regulating rap small g-proteins in time and space. Trends Cell Biol 21:615–623
Ohba Y, Kurokawa K, Matsuda M (2003) Mechanism of the spatio-temporal regulation of ras and rap1. EMBO J 22:859–869
Retta SF, Balzac F, Avolio M (2006) Rap1: a turnabout for the crosstalk between cadherins and integrins. Eur J Cell Biol 85:283–293
Bivona TG, Wiener HH, Ahearn IM et al (2004) Rap1 up-regulation and activation on plasma membrane regulates t cell adhesion. J Cell Biol 164:461–470
Bos JL, de Bruyn K, Enserink J et al (2003) The role of rap1 in integrin-mediated cell adhesion. Biochem Soc Trans 31:83–86
Dustin ML, Bivona TG, Philips MR (2004) Membranes as messengers in t cell adhesion signaling. Nat Immunol 5:363–372
Katagiri K, Maeda A, Shimonaka M et al (2003) Rapl, a rap1-binding molecule that mediates rap1-induced adhesion through spatial regulation of lfa-1. Nat Immunol 4:741–748
Han J, Lim CJ, Watanabe N et al (2006) Reconstructing and deconstructing agonist-induced activation of integrin alphaiibbeta3. Curr Biol 16:1796–1806
Kim C, Ye F, Ginsberg MH (2011) Regulation of integrin activation. Annu Rev Cell Dev Biol 27:321–345
Balzac F, Avolio M, Degani S et al (2005) E-cadherin endocytosis regulates the activity of rap1: a traffic light gtpase at the crossroads between cadherin and integrin function. J Cell Sci 118:4765–4783
Asuri S, Yan J, Paranavitana NC et al (2008) E-cadherin dis-engagement activates the rap1 gtpase. J Cell Biochem 105:1027–1037
Hogan C, Serpente N, Cogram P et al (2004) Rap1 regulates the formation of e-cadherin-based cell–cell contacts. Mol Cell Biol 24:6690–6700
Rottner K, Krause M, Gimona M et al (2001) Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (vasp) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and vasp in focal adhesions. Mol Biol Cell 12:3103–3113
Zaidel-Bar R, Cohen M, Addadi L et al (2004) Hierarchical assembly of cell–matrix adhesion complexes. Biochem Soc Trans 32: 416–420
Ambesi-Impiombato FS, Parks LA, Coon HG (1980) Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc Natl Acad Sci U S A 77:3455–3459
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Goitre, L., Cutano, V., Retta, S.F. (2014). Fluorescence Microscopy Study of Rap1 Subcellular Localization. In: Trabalzini, L., Retta, S. (eds) Ras Signaling. Methods in Molecular Biology, vol 1120. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-791-4_13
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DOI: https://doi.org/10.1007/978-1-62703-791-4_13
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-791-4
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