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Sirtuins pp 285–302Cite as

Circadian Measurements of Sirtuin Biology

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1077))

Abstract

Many of our behavioral and physiological processes display daily oscillations that are under the control of the circadian clock. The core molecular clock network is present in both the brain and peripheral tissues and is composed of a complex series of interlocking transcriptional/translational feedback loops that oscillate with a periodicity of ~24 h. Recent evidence has implicated NAD+ biosynthesis and the sirtuin family of NAD+-dependent protein deacetylases as part of a novel feedback loop within the core clock network, findings which underscore the importance of taking circadian timing into consideration when designing and interpreting metabolic studies, particularly in regard to sirtuin biology. Thus, this chapter introduces both in vivo and in vitro circadian methods to analyze various sirtuin-related endpoints across the light–dark cycle and discusses the transcriptional, biochemical, and physiological outputs of the clock.

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Authors and Affiliations

  1. Division of Endocrinology, Metabolism and Molecular Medicine, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA

    Kathryn Moynihan Ramsey, Alison H. Affinati, Clara B. Peek, Biliana Marcheva, Hee-Kyung Hong & Joseph Bass

  2. Department of Neurobiology, Northwestern University, Evanston, IL, USA

    Kathryn Moynihan Ramsey, Alison H. Affinati, Clara B. Peek, Biliana Marcheva, Hee-Kyung Hong & Joseph Bass

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  1. Kathryn Moynihan Ramsey
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  2. Alison H. Affinati
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  3. Clara B. Peek
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  4. Biliana Marcheva
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  5. Hee-Kyung Hong
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  6. Joseph Bass
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Editor information

Editors and Affiliations

  1. Duke University Medical Center Institute of Molecular Physiology, Durham, North Carolina, USA

    Matthew D. Hirschey

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Ramsey, K.M., Affinati, A.H., Peek, C.B., Marcheva, B., Hong, HK., Bass, J. (2013). Circadian Measurements of Sirtuin Biology. In: Hirschey, M. (eds) Sirtuins. Methods in Molecular Biology, vol 1077. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-637-5_19

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  • DOI: https://doi.org/10.1007/978-1-62703-637-5_19

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  • Publisher Name: Humana Press, Totowa, NJ

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