Abstract
Microglia, neurons, and macroglia (astrocytes and oligodendrocytes) are the major cell types in the central nervous system. In the past decades, primary microglia-enriched cultures have been widely used to study the biological functions of microglia in vitro. In order to study the interactions between microglia and other brain cells, neuron–glia, neuron–microglia, and mixed glia cultures were developed. The aim of this chapter is to provide basic and adaptable protocols for the preparation of these microglia-containing primary cultures from rodent. Meanwhile, we also want to provide a collection of tips from our collective experiences doing primary brain cell cultures.
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Costero J (1930) Estudie del compotamento de la microlgia cultivade on vetro. Datos concernientes a su histogenesis. Mem R Soc cep Hist nat 14:125–182
Kettenmann H, Hanisch UK, Noda M et al (2011) Physiology of microglia. Physiol Rev 91(2):461–553
Giulian D, Baker TJ (1986) Characterization of ameboid microglia isolated from developing mammalian brain. J Neurosci 6(8):2163–2178
Gao HM, Hong JS, Zhang W et al (2002) Distinct role for microglia in rotenone-induced degeneration of dopaminergic neurons. J Neurosci 22(3):782–790
Gao HM, Jiang J, Wilson B et al (2002) Microglial activation-mediated delayed and progressive degeneration of rat nigral dopaminergic neurons: relevance to Parkinson's disease. J Neurochem 81(6):1285–1297
Qin L, Liu Y, Cooper C et al (2002) Microglia enhance beta-amyloid peptide-induced toxicity in cortical and mesencephalic neurons by producing reactive oxygen species. J Neurochem 83(4):973–983
Gao HM, Hong JS, Zhang W et al (2003) Synergistic dopaminergic neurotoxicity of the pesticide rotenone and inflammogen lipopolysaccharide: relevance to the etiology of Parkinson's disease. J Neurosci 23(4):1228–1236
Chang RC, Chen W, Hudson P et al (2001) Neurons reduce glial responses to lipopolysaccharide (LPS) and prevent injury of microglial cells from over-activation by LPS. J Neurochem 76(4):1042–1049
Liu B, Du L, Hong JS (2000) Naloxone protects rat dopaminergic neurons against inflammatory damage through inhibition of microglia activation and superoxide generation. J Pharmacol Exp Ther 293(2):607–617
Paxinos G, Tork I, Tecott LH et al (1991) Atlas of the developing rat brain. Academic, San Diego
Hong JS, Wood PL, Gillin JC et al (1980) Changes of hippocampal Met-enkephalin content after recurrent motor seizures. Nature 285(5762):231–232
Butler H, Juurlink BHJ (1987) An atlas for staging mammalian and chick embryos, 1st edn. CRC, Boca Raton
Torres EM, Weyrauch UM, Sutcliffe R et al (2008) A rat embryo staging scale for the generation of donor tissue for neural transplantation. Cell Transplant 17(5):535–542
Acknowledgements
This research was supported [in part] by the Intramural Research Program of the NIH, National Institute of Environmental Health Sciences. We would like to acknowledge Dr. Bin Liu for his contribution in developing these protocols.
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Chen, SH., Oyarzabal, E.A., Hong, JS. (2013). Preparation of Rodent Primary Cultures for Neuron–Glia, Mixed Glia, Enriched Microglia, and Reconstituted Cultures with Microglia. In: Joseph, B., Venero, J. (eds) Microglia. Methods in Molecular Biology, vol 1041. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-520-0_21
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DOI: https://doi.org/10.1007/978-1-62703-520-0_21
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-519-4
Online ISBN: 978-1-62703-520-0
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