Overview
- Serves as an essential collection of protocols from leading authors in a variety of disciplines
- Features detailed, lab-ready methodologies
- Includes expert tips and key implementation advice to ensure successful results
Part of the book series: Methods in Molecular Biology (MIMB, volume 1032)
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About this book
Over the last half century, a dramatic increase in allergic diseases has been observed throughout industrialized nations, which has resulted in significant worldwide socio-economic challenges. In Mouse Models of Allergic Disease: Methods and Protocols, a wide range of expert contributors provide detailed protocols for the design and execution of experiments to thoroughly analyze critical elements associated with a diverse range of allergic diseases, all through the lens of mouse models that accurately recapitulate clinically relevant aspects of the respective human disease. The volume opens with a section featuring techniques essential for effective ex vivo cell isolation and evaluation of specific cell types relevant to a diverse range of allergic diseases, and the book then moves on to cover in vivo protocols to evaluate prevalent mouse models of human allergic diseases, including mouse models of systemic anaphylaxis, contact hypersensitivity, allergic rhinitis, and asthma, as well as a collection of chapters on in vivo and ex vivo protocols used to assess indirect mediators of allergic diseases, such as the nervous system, non-hematopoietic cells, and the composition of the gut microbiome. Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Timely and authoritative, Mouse Models of Allergic Disease: Methods and Protocols serves as an essential collection of protocols that allow both novice and expert researchers the ability to accurately develop, evaluate, and characterize the mechanisms associated with these disorders.
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Keywords
Table of contents (24 protocols)
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Front Matter
Reviews
“This book is a collection of beautifully written chapters that are suitable to explore in-depth use of mouse models to understand allergic disease. … This is a must read book for trainees, clinicians and researchers engaged in the study of allergy related manifestations. Undoubtedly, this book will provide an in-depth understanding regarding the use of mouse in allergic research. Using this invaluable text, various key phenomena behind allergic reactions can be explored.” (Sandeep Kumar, World Allergy Organization, worldallergy.org, May, 2015)
Editors and Affiliations
Bibliographic Information
Book Title: Mouse Models of Allergic Disease
Book Subtitle: Methods and Protocols
Editors: Irving C. Allen
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-496-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-495-1Published: 14 August 2013
Softcover ISBN: 978-1-4939-6020-0Published: 23 August 2016
eBook ISBN: 978-1-62703-496-8Published: 15 August 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 326
Number of Illustrations: 24 b/w illustrations, 27 illustrations in colour
Topics: Immunology