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Transplantation Immunology pp 353–358Cite as

Detection of Intracellular Cytokines

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1034))

Abstract

The intracellular cytokine method allows for a multiparametric readout by flow cytometry with precise phenotyping of the responding T cells. The intracellular cytokine staining of cells that have been fixed and permeabilized following a short-term activation and staining with antibodies to cell surface markers allows for identification of the cellular origin of the cytokine accumulated product.

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References

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Author information

Authors and Affiliations

  1. HLA Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, USA

    Nancy L. Reinsmoen & Chih-Hung Lai

Authors
  1. Nancy L. Reinsmoen
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  2. Chih-Hung Lai
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Editor information

Editors and Affiliations

  1. , Immunogenetics Laboratory, Johns Hopkins University School of Medic, E. Monument Street 2041, Baltimore, 21205, Maryland, USA

    Andrea A. Zachary

  2. , Immunogenetics Laboratory, Johns Hopkins University School of Medic, E. Monument Street 2041, Baltimore, 21205, Maryland, USA

    Mary S. Leffell

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© 2013 Springer Science+Business Media, LLC

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Reinsmoen, N.L., Lai, CH. (2013). Detection of Intracellular Cytokines. In: Zachary, A., Leffell, M. (eds) Transplantation Immunology. Methods in Molecular Biology, vol 1034. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-493-7_22

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  • DOI: https://doi.org/10.1007/978-1-62703-493-7_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-492-0

  • Online ISBN: 978-1-62703-493-7

  • eBook Packages: Springer Protocols

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