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Gold Nanoparticles and Oxidative Stress in the Blue Mussel, Mytilus edulis

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Book cover Oxidative Stress and Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1028))

Abstract

Relatively little is known about how gold nanoparticles (GNP) might interact in vivo with aquatic organisms. Gold nanoparticles (GNP) of defined average diameter may be synthesized and used to challenge test organisms held in aquaria. The blue mussel, Mytilus edulis, is a popular sentinel species in environmental toxicology. This chapter describes how mussels exposed to GNP (average diameter ~5 nm) may be dissected and extracts of digestive gland can be analyzed for oxidative stress. Protein thiols are labeled with 5′-iodoacetamide-fluorescein (IAF), and proteins are separated in one-dimensional electrophoresis. After scanning for IAF-associated fluorescence, gels are stained with colloidal coomassie. A ratio of fluorescence to protein stain is calculated revealing that thiol oxidation increases with GNP treatment.

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Abbreviations

1-DE:

One-dimensional electrophoresis

DMSO:

Dimethyl sulfoxide

EDTA:

Ethylenediaminetetraacetic acid

GSH:

Reduced glutathione

GNP:

Gold nanoparticle

IAF:

5′-Iodoacetamide-fluorescein

TEMED:

N,N,N,N′-tetramethyl-ethylenediamine

PMSF:

Phenylmethylsulfonyl fluoride

ROS:

Reactive oxygen species

SDS PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

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Author information

Authors and Affiliations

  1. Proteomics Research Laboratory, Department of Biochemistry, University College Cork, Cork, Ireland

    Sara Tedesco, Irene O’Donovan, Sarah Keane & David Sheehan

  2. Tyndall National Laboratory, University College Cork, Cork, Ireland

    Hugh Doyle & Daniela Iacopino

Authors
  1. Sara Tedesco
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  2. Hugh Doyle
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  3. Daniela Iacopino
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  4. Irene O’Donovan
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  5. Sarah Keane
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  6. David Sheehan
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Editor information

Editors and Affiliations

  1. University of Florida, n/a, Gainsville, 32611, Florida, USA

    Donald Armstrong

  2. , Pharmaceutical Research Institute, Albany College of Pharmacy, Discovery Lane 1, Rensselaer, 12144, USA

    Dhruba J Bharali

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Tedesco, S., Doyle, H., Iacopino, D., O’Donovan, I., Keane, S., Sheehan, D. (2013). Gold Nanoparticles and Oxidative Stress in the Blue Mussel, Mytilus edulis . In: Armstrong, D., Bharali, D. (eds) Oxidative Stress and Nanotechnology. Methods in Molecular Biology, vol 1028. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-475-3_12

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  • DOI: https://doi.org/10.1007/978-1-62703-475-3_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-474-6

  • Online ISBN: 978-1-62703-475-3

  • eBook Packages: Springer Protocols

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