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Protein-Ligand Interactions pp 63–99Cite as

Measurement of Protein–Ligand Complex Formation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1008))

Abstract

Experimental approaches to detect, measure, and quantify protein–ligand binding, along with their theoretical bases, are described. A range of methods for detection of protein–ligand interactions is summarized. Specific protocols are provided for a nonequilibrium procedure pull-down assay, for an equilibrium direct binding method and its modification into a competition-based measurement and for steady-state measurements based on the effects of ligands on enzyme catalysis.

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Acknowledgements

We gratefully acknowledge the expert advice and information provided by Pirthipal Singh (Singh Consultancy) in the preparation of this chapter.

Author information

Authors and Affiliations

  1. Biomolecular Interactions Consultancy, Hertford, UK

    Peter N. Lowe

  2. Department of Biological Sciences, Institute of Structural and Molecular Biology, Birkbeck, University of London, London, UK

    Cara K. Vaughan

  3. ISMB Biophysics Centre, Institute of Structural and Molecular Biology, Birkbeck, University of London, London, UK

    Tina Daviter

Authors
  1. Peter N. Lowe
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  2. Cara K. Vaughan
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  3. Tina Daviter
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Editor information

Editors and Affiliations

  1. Birbeck, University of London, ISMB Biophysics Centre, Institute of Str, Malet Street, London, WC1E 7HX, United Kingdom

    Mark A. Williams

  2. Birkbeck, University of London, School of Crystallography, ISMB Biophysics Centre, Institute of Str, Malet Street, London, WC1E 7HX, United Kingdom

    Tina Daviter

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Lowe, P.N., Vaughan, C.K., Daviter, T. (2013). Measurement of Protein–Ligand Complex Formation. In: Williams, M., Daviter, T. (eds) Protein-Ligand Interactions. Methods in Molecular Biology, vol 1008. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-398-5_3

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  • DOI: https://doi.org/10.1007/978-1-62703-398-5_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-397-8

  • Online ISBN: 978-1-62703-398-5

  • eBook Packages: Springer Protocols

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