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Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact

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Book cover The Cytoskeleton

Part of the book series: Neuromethods ((NM,volume 79))

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Abstract

The cytoskeleton constitutes a dynamic intracellular framework for inbound and outbound trafficking of cell organelles and is responsible for cell shaping including cell division. While transport in neuronal compartments has extensively been conquered in particular the shipping of cargo vesicles along microtubules for support of chemical synapses, the supply of molecular constituents to electrical synapses is still enigmatic. Here we describe protocols for proteomic analysis of cytoskeletal elements involved in the trafficking of connexin43, the major gap junction protein expressed in astrocytes. Methods described here offer hands-on instructions for further successful exploration of the cytoskeletal and its interaction with gap junction proteins.

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Authors and Affiliations

  1. Department of Neuroanatomy and Molecular Brain Research, Faculty of Medicine, Ruhr-University Bochum, Bochum, Germany

    Georg Zoidl & Rolf Dermietzel

  2. Department of Psychology, York University, Toronto, ON, Canada

    Georg Zoidl

  3. Department of Neuroanatomy and Molecular Brain Research, Ruhr University of Bochum, Bochum, Germany

    Stefan Olk & Andrey Tuchinowitz

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  1. Georg Zoidl
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  2. Stefan Olk
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  3. Andrey Tuchinowitz
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  4. Rolf Dermietzel
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Corresponding author

Correspondence to Rolf Dermietzel .

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Editors and Affiliations

  1. Faculty of Medicine, Neuroanatomy & Molecular Brain Research, Ruhr-Universität Bochum, Universitätsstrasse 150, Bochum, 44801, Germany

    Rolf Dermietzel

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Zoidl, G., Olk, S., Tuchinowitz, A., Dermietzel, R. (2013). Cell-to-Cell Communication in Astroglia and the Cytoskeletal Impact. In: Dermietzel, R. (eds) The Cytoskeleton. Neuromethods, vol 79. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-266-7_12

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  • DOI: https://doi.org/10.1007/978-1-62703-266-7_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-265-0

  • Online ISBN: 978-1-62703-266-7

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