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Use of Macrocyclic Antibiotics as the Chiral Selectors in Capillary Electrophoresis

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Chiral Separations

Part of the book series: Methods in Molecular Biology ((MIMB,volume 970))

Abstract

Macrocyclic antibiotics, especially vancomycin, represent a class of versatile chiral selectors for enantioseparations by capillary electrophoresis (CE). In this chapter, we describe the protocol for performing CE enantioseparations with vancomycin as the chiral selector. Dynamic coating of the capillary with polymers including the positively charged polyelectrolyte hexadimethrine bromide or electro-neutral poly(dimethylacrylamide) proved to be very useful in order to reduce the adsorption of vancomycin onto the capillary wall resulting in an improved separation efficiency. The partial filling technique is employed for improvement of the detection sensitivity. Utilization of these techniques makes the CE enantioseparation with vancomycin more practical and robust.

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Acknowledgments

The financial support by the National Natural Science Foundations of China (21175146, 20975109, 90713021, and 20675086) and the opening foundations from the National Key Laboratory of Organic Biochemistry are gratefully acknowledged.

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Authors and Affiliations

  1. Chinese Academy of Sciences, Shanghai Institute of Organic Chemistry, Shanghai, China

    Chengke Li & Jingwu Kang

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  1. Chengke Li
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  2. Jingwu Kang
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Correspondence to Jingwu Kang .

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Editors and Affiliations

  1. , Department of Pharmaceutical Chemistry, Friedrich-Schiller-University Jena, Philosophenweg 14, Jena, 07743, Germany

    Gerhard K. E. Scriba

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Li, C., Kang, J. (2013). Use of Macrocyclic Antibiotics as the Chiral Selectors in Capillary Electrophoresis. In: Scriba, G. (eds) Chiral Separations. Methods in Molecular Biology, vol 970. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-263-6_20

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  • DOI: https://doi.org/10.1007/978-1-62703-263-6_20

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-262-9

  • Online ISBN: 978-1-62703-263-6

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