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Micropropagation of Vaccinium sp. by In Vitro Axillary Shoot Proliferation

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Protocols for Micropropagation of Selected Economically-Important Horticultural Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 994))

Abstract

The Vaccinium genus contains several valuable fruit and ornamental species, among others: highbush blueberry (Vaccinium × corymbosum L.), cranberry (Vaccinium macrocarpon Ait.), and lingonberry (Vaccinium vitis-idaea L.). In some most popular and valuable cultivars, the conventional propagation methods, exploiting hard or soft wood cuttings, are inefficient. The demand for nursery plants could be fulfilled only by micropropagation. In principle cultivars are propagated in vitro through similar three-stage method, based on subculture of shoot explants on different culture media supplemented with IAA (0–4 mg/L) and 2iP (5–10 mg/L), and rooting shoots in vivo. The obtained plantlets are transferred to peat substrate and grown in the glasshouse until the end of growing period. The development of adventitious shoots should be monitored and controlled during in vitro stages. Many clones have specific requirements for growing conditions and/or are recalcitrant.

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Authors and Affiliations

  1. Faculty of Biology and Agriculture, Department of Plant Physiology and Biotechnology, University of Rzeszów, Rzeszów, Poland

    Wojciech Litwińczuk

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  1. Wojciech Litwińczuk
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Correspondence to Wojciech Litwińczuk .

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Editors and Affiliations

  1. , IVALSA/Istituto per la Valorizzazione, National Research Council (CNR), Polo Scientifico, via Madonna del Piano n. 10, Sesto Fiorentino, Florence, 50019, Italy

    Maurizio Lambardi

  2. , Istituto per la Valorizzazione del Legno, National Research Council (CNR), n/a, Florence, n/a, Italy

    Elif Aylin Ozudogru

  3. , Department of Agricultural, University of Helsinki, 5 Latokartanonkaari Room C 130, Helsinki, 00014, Finland

    Shri Mohan Jain

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Litwińczuk, W. (2012). Micropropagation of Vaccinium sp. by In Vitro Axillary Shoot Proliferation. In: Lambardi, M., Ozudogru, E., Jain, S. (eds) Protocols for Micropropagation of Selected Economically-Important Horticultural Plants. Methods in Molecular Biology, vol 994. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-074-8_5

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  • DOI: https://doi.org/10.1007/978-1-62703-074-8_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-073-1

  • Online ISBN: 978-1-62703-074-8

  • eBook Packages: Springer Protocols

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