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Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues

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Nanoparticles in Biology and Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 906))

Abstract

Like most of the new technologies designed to interact with biological systems, the applications of ­nanomaterials needs a proper assessment for their potential impacts. It is only through addressing the issues raised by toxicological studies that nanotechnology will be able to acquire its full potential. Here, we describe the detailed protocols to study the responses of plant cells to their exposure to nanoparticles, including viability, oxidative stress detection, and reactive oxygen species enzymatic detoxification, as well as particle uptake.

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Acknowledgments

This work was supported by the project “Development of ultra-sensitive detection methods and plant nano-vaccines for the fungi Fusarium spp. using nanotechnological devices” Iberian Capacitation Program in Nanotechnologies: Call 2006/2007. This work was also supported by Fundação para a Ciência e a Tecnologia through grant # PEst-OE/EQB/LA0004/2011.

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Authors and Affiliations

  1. Diagnostic, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157, Oeiras, Portugal

    Ana Raquel Santos

  2. Organic Synthesis Laboratory, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, Oeiras, Portugal

    Ana Sofia Miguel

  3. Biomolecular Diagnostics Laboratory, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal

    Ana Sofia Miguel

  4. Plant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal

    Pedro Fevereiro

  5. Biomolecular Diagnostics Laboratory, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da Republica, Oeiras, Portugal

    Abel Oliva

Authors
  1. Ana Raquel Santos
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  2. Ana Sofia Miguel
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  3. Pedro Fevereiro
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  4. Abel Oliva
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Corresponding author

Correspondence to Ana Raquel Santos .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, Royal Holloway University of London, Egham Hill, Egham, Surrey, TW20 0EX, United Kingdom

    Mikhail Soloviev

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Santos, A.R., Miguel, A.S., Fevereiro, P., Oliva, A. (2012). Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_36

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  • DOI: https://doi.org/10.1007/978-1-61779-953-2_36

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-952-5

  • Online ISBN: 978-1-61779-953-2

  • eBook Packages: Springer Protocols

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