Abstract
Quantum dots (QDs) are semiconductor nanocrystals with unique photo-physical properties. QDs are brightly fluorescent and photostable, and therefore represent a novel class of fluorescence probes for bio-imaging and cell monitoring. There are different techniques for labeling cells with QDs. Moreover, there is evidence that optical properties of the QD can be changed as a result of interaction with live cells. This manuscript describes a simple method for labeling of rat endothelial progenitor cells using ODs and for characterization of their optical properties.
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Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307:538–544
Wang Y, Chen L (2011) Quantum dots, lighting up the research and development of nanomedicine. Nanomedicine. doi:10.1016/j.nano.2010.1012.1006
Noh YW, Lim YT, Chung BH (2008) Noninvasive imaging of dendritic cell migration into lymph nodes using near-infrared fluorescent semiconductor nanocrystals. FASEB J 22:3908–3918
Slotkin JR, Chakrabarti L, Dai HN, Carney RS, Hirata T, Bregman BS, Gallicano GI, Corbin JG, Haydar TF (2007) In vivo quantum dot labeling of mammalian stem and progenitor cells. Dev Dyn 236:3393–3401
Jayagopal A, Su YR, Blakemore JL, Linton MF, Fazio S, Haselton FR (2009) Quantum dot mediated imaging of atherosclerosis. Nanotechnology 20:165102
Yukawa H, Mizufune S, Mamori C, Kagami Y, Oishi K, Kaji N, Okamoto Y, Takeshi M, Noguchi H, Baba Y, Hamaguchi M, Hamajima N, Hayashi S (2009) Quantum dots for labeling adipose tissue-derived stem cells. Cell Transplant 18(5):591–599
Rosen AB, Kelly DJ, Schuldt AJ, Lu J, Potapova IA, Doronin SV, Robichaud KJ, Robinson RB, Rosen MR, Brink PR, Gaudette GR, Cohen IS (2007) Finding fluorescent needles in the cardiac haystack: tracking human mesenchymal stem cells labeled with quantum dots for quantitative in vivo three-dimensional fluorescence analysis. Stem Cells 25:2128–2138
Molnar M, Friberg P, Fu Y, Brisslert M, Adams M, Chen Y (2010) Effects of quantum dot labeling on endothelial progenitor cell function and viability. Cell Med B Cell Transplant 1:105–112
Zhang LW, Monteiro-Riviere NA (2009) Mechanisms of quantum dot nanoparticle cellular uptake. Toxicol Sci 110:138–155
Molnar M, Fu Y, Friberg P, Chen Y (2010) Optical characterization of colloidal CdSe quantum dots in endothelial progenitor cells. J Nanobiotechnology 8:2
Riegler J, Ditengou F, Palme K, Nann T (2008) Blue shift of CdSe/ZnS nanocrystal-labels upon DNA-hybridization. J Nanobiotechnology 6:7
Acknowledgments
This study was supported by VINNOVA (Project number P35914-1) and Wilhelm & Martina Lundgren Foundation.
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Chen, Y. (2012). Quantum Dots for Labeling Live Cells. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_14
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DOI: https://doi.org/10.1007/978-1-61779-953-2_14
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Publisher Name: Humana Press, Totowa, NJ
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