Skip to main content

Quantum Dots for Labeling Live Cells

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 906))

Abstract

Quantum dots (QDs) are semiconductor nanocrystals with unique photo-physical properties. QDs are brightly fluorescent and photostable, and therefore represent a novel class of fluorescence probes for bio-imaging and cell monitoring. There are different techniques for labeling cells with QDs. Moreover, there is evidence that optical properties of the QD can be changed as a result of interaction with live cells. This manuscript describes a simple method for labeling of rat endothelial progenitor cells using ODs and for characterization of their optical properties.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307:538–544

    Article  PubMed  CAS  Google Scholar 

  2. Wang Y, Chen L (2011) Quantum dots, lighting up the research and development of ­nanomedicine. Nanomedicine. doi:10.1016/j.nano.2010.1012.1006

  3. Noh YW, Lim YT, Chung BH (2008) Noninvasive imaging of dendritic cell migration into lymph nodes using near-infrared fluorescent semiconductor nanocrystals. FASEB J 22:3908–3918

    Article  PubMed  CAS  Google Scholar 

  4. Slotkin JR, Chakrabarti L, Dai HN, Carney RS, Hirata T, Bregman BS, Gallicano GI, Corbin JG, Haydar TF (2007) In vivo quantum dot labeling of mammalian stem and progenitor cells. Dev Dyn 236:3393–3401

    Article  PubMed  CAS  Google Scholar 

  5. Jayagopal A, Su YR, Blakemore JL, Linton MF, Fazio S, Haselton FR (2009) Quantum dot mediated imaging of atherosclerosis. Nanotechnology 20:165102

    Article  PubMed  Google Scholar 

  6. Yukawa H, Mizufune S, Mamori C, Kagami Y, Oishi K, Kaji N, Okamoto Y, Takeshi M, Noguchi H, Baba Y, Hamaguchi M, Hamajima N, Hayashi S (2009) Quantum dots for labeling adipose tissue-derived stem cells. Cell Transplant 18(5):591–599

    PubMed  Google Scholar 

  7. Rosen AB, Kelly DJ, Schuldt AJ, Lu J, Potapova IA, Doronin SV, Robichaud KJ, Robinson RB, Rosen MR, Brink PR, Gaudette GR, Cohen IS (2007) Finding fluorescent needles in the cardiac haystack: tracking human mesenchymal stem cells labeled with quantum dots for quantitative in vivo three-dimensional fluorescence analysis. Stem Cells 25:2128–2138

    Article  PubMed  CAS  Google Scholar 

  8. Molnar M, Friberg P, Fu Y, Brisslert M, Adams M, Chen Y (2010) Effects of quantum dot labeling on endothelial progenitor cell function and viability. Cell Med B Cell Transplant 1:105–112

    Google Scholar 

  9. Zhang LW, Monteiro-Riviere NA (2009) Mechanisms of quantum dot nanoparticle cellular uptake. Toxicol Sci 110:138–155

    Article  PubMed  CAS  Google Scholar 

  10. Molnar M, Fu Y, Friberg P, Chen Y (2010) Optical characterization of colloidal CdSe quantum dots in endothelial progenitor cells. J Nanobiotechnology 8:2

    Article  PubMed  Google Scholar 

  11. Riegler J, Ditengou F, Palme K, Nann T (2008) Blue shift of CdSe/ZnS nanocrystal-labels upon DNA-hybridization. J Nanobiotechnology 6:7

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by VINNOVA (Project number P35914-1) and Wilhelm & Martina Lundgren Foundation.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yun Chen .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Chen, Y. (2012). Quantum Dots for Labeling Live Cells. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-953-2_14

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-952-5

  • Online ISBN: 978-1-61779-953-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics