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Immunogenetics pp 173–182Cite as

Genotyping of Single Nucleotide Polymorphisms by 5′ Nuclease Allelic Discrimination

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 882))

Abstract

Real-time quantitative PCR is an efficient method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). In this chapter, we describe the 5′ nuclease allelic discrimination assay for genotyping biallelic SNPs.

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Author information

Authors and Affiliations

  1. Fred Hutchinson Cancer Research Centre, Seattle, WA, USA

    Mari Malkki & Effie W. Petersdorf

Authors
  1. Mari Malkki
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  2. Effie W. Petersdorf
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Corresponding author

Correspondence to Effie W. Petersdorf .

Editor information

Editors and Affiliations

  1. PathWest Laboratory Medicine, Department of Clinical Immunology, Royal Perth Hospital, Wellington Street, Perth, 6000, Australia

    Frank T. Christiansen

  2. Australian Red Cross Blood Service, National Transplant Services, The Royal Melbourne Hospital, 150-154 Batman Street, Melbourne VIC, 3000, Australia

    Brian D. Tait

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Malkki, M., Petersdorf, E.W. (2012). Genotyping of Single Nucleotide Polymorphisms by 5′ Nuclease Allelic Discrimination. In: Christiansen, F., Tait, B. (eds) Immunogenetics. Methods in Molecular Biology, vol 882. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-842-9_10

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  • DOI: https://doi.org/10.1007/978-1-61779-842-9_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-841-2

  • Online ISBN: 978-1-61779-842-9

  • eBook Packages: Springer Protocols

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