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Somatic Stem Cells

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Shree Ram Singh

    1. Mouse Cancer Genetics Program, National Cancer Institute, Frederick, USA

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  • Presents selected genetic, molecular, and cellular techniques used in somatic stem cell reserach and its clinical application
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 879)

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About this book

 Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Protocols presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Through and intuitive, Somatic Stem Cells: Methods and Protocols seeks to provides scientists with the fundamental techniques of stem cell research and its potential application in regenerative medicine.

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xvi
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Current Thoughts on the Therapeutic Potential of Stem Cell

      • Pranela Rameshwar
      Pages 3-26

  3. Stem Cell Study in Lower Organism

    1. Front Matter

      Pages 27-27
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  4. Stem Cell Study in Lower Organism

    1. A Unique FACS Method to Isolate Stem Cells in Planarian

      • Tetsutaro Hayashi, Kiyokazu Agata
      Pages 29-37

    2. Identification of Neural Stem Cells in the Drosophila Larval Brain

      • Mo Weng, Hideyuki Komori, Cheng-Yu Lee
      Pages 39-46

    3. Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut

      • Shree Ram Singh, Manoj K. Mishra, Madhuri Kango-Singh, Steven X. Hou
      Pages 47-69

  5. Stem Cell Study in Murine and Human Model

    1. Front Matter

      Pages 71-71
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    2. Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial Cells

      • Daniel Croagh, Rick Redvers, Wayne A. Phillips, Pritinder Kaur
      Pages 73-88

    3. Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine Intestine

      • A. D. Gracz, B. J. Puthoff, S. T. Magness
      Pages 89-107

    4. Isolation and Characterization of Distal Lung Progenitor Cells

      • Barbara Driscoll, Alex Kikuchi, Allison N. Lau, Jooeun Lee, Raghava Reddy, Edwin Jesudason et al.
      Pages 109-122

    5. Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Progenitor Cells

      • Kristbjorn Orri Gudmundsson, Steven W. Stull, Jonathan R. Keller
      Pages 123-133

    6. Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells

      • Weihui Liu, Nan You, Kefeng Dou
      Pages 135-146

    7. Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases

      • Laurent Lescaudron, C. Boyer, Virginie Bonnamain, K. D. Fink, X. Lévêque, J. Rossignol et al.
      Pages 147-164

    8. Functional Identification of Neural Stem Cell-Derived Oligodendrocytes

      • Sofia Grade, Fabienne Agasse, Liliana Bernardino, João O. Malva
      Pages 165-178

    9. Stem/Progenitor Cells in Murine Mammary Gland: Isolation and Functional Characterization

      • Abhik Bandyopadhyay, Qiaoxiang Dong, Lu-Zhe Sun
      Pages 179-193

    10. A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-cultures

      • Steven J. Greco, Shyam A. Patel, Pranela Rameshwar
      Pages 195-201

    11. Isolation, Culture, and Osteogenic/Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

      • Susanne Grässel, Sabine Stöckl, Zsuzsa Jenei-Lanzl
      Pages 203-267

    12. Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue

      • Andrew C. Boquest, Philippe Collas
      Pages 269-278

    13. Collection, Processing, and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine

      • Michael S. Badowski, David T. Harris
      Pages 279-290
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Editors and Affiliations

  • Mouse Cancer Genetics Program, National Cancer Institute, Frederick, USA

    Shree Ram Singh

Bibliographic Information

  • Book Title: Somatic Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Shree Ram Singh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-815-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-814-6Published: 23 May 2012

  • Softcover ISBN: 978-1-4939-6234-1Published: 23 August 2016

  • eBook ISBN: 978-1-61779-815-3Published: 19 May 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 546

  • Topics: Cell Biology, Stem Cells

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