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Laser Speckle Imaging

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Animal Models of Acute Neurological Injuries II

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Impaired cortical cerebral blood flow (CBF) is a main indicator for hemodynamic perfusion deficits of the brain. After aneurysmal subarachnoid hemorrhage (SAH), vasospasm may cause hemodynamic hypoperfusion and subsequent cortical border zone infarction. In order to provide dynamic real-time assessment of cortical CBF after experimental SAH, Laser Speckle Contrast Analysis (LASCA) serves as a novel tool for semiquantitative and noninvasive cortical perfusion measurement with high spatial and temporal resolution. This chapter describes the principle of LASCA, technique and procedure, pitfalls and limitations of the technique on the basis of a mouse model of aneurysmal SAH.

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Author information

Authors and Affiliations

  1. Neurochirurgische Klinik, Campus Virchow Klinikum, Charité Universitätsmedizin Berlin, Berlin, Germany

    Nils Hecht, Ulf C. Schneider, Johannes Woitzik & Peter Vajkoczy

Authors
  1. Nils Hecht
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  2. Ulf C. Schneider
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  3. Johannes Woitzik
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  4. Peter Vajkoczy
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Corresponding author

Correspondence to Nils Hecht .

Editor information

Editors and Affiliations

  1. School of Medicine, University of Pittsburgh, Terrace St. 3500, Pittsburgh, 15213, Pennsylvania, USA

    Jun Chen

  2. School of Medicine, Stark Neurosciences Res. Inst., Indiana University, W. Walnut St. 950, Indianapolis, 46202, Indiana, USA

    Xiao-Ming Xu

  3. School of Medicine, Department of Anatomy & Cell Biology, Indiana University, Barnhill Dr. 635, Indianapolis, 46202, Indiana, USA

    Zao C. Xu

  4. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    John H. Zhang

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Hecht, N., Schneider, U.C., Woitzik, J., Vajkoczy, P. (2012). Laser Speckle Imaging. In: Chen, J., Xu, XM., Xu, Z., Zhang, J. (eds) Animal Models of Acute Neurological Injuries II. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-576-3_41

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  • DOI: https://doi.org/10.1007/978-1-61779-576-3_41

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-575-6

  • Online ISBN: 978-1-61779-576-3

  • eBook Packages: Springer Protocols

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