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Leucocytes

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Robert B. Ashman

    1. School of Dentistry, University of Queensland, Brisbane, Australia

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  • Provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 844)

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About this book

The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability to modify the genome in vivo, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. In, Leucocytes: Methods and Protocols provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Leucocytes: Methods and Protocols seeks to aid scientists in further study into the immune system and leucocytes.

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. ENU-Based Phenotype-Driven Screening

    • Vera M. Ripoll, Philip L. Kong, Paul K. Potter
    Pages 1-13

  3. Detection and Quantification of Cytokines and Other Biomarkers

    • Evan L. Chiswick, Elizabeth Duffy, Brian Japp, Daniel Remick
    Pages 15-30

  4. Flow Cytometry Analysis of Cell Cycling and Proliferation in Mouse Hematopoietic Stem and Progenitor Cells

    • Valérie Barbier, Bianca Nowlan, Jean-Pierre Lévesque, Ingrid G. Winkler
    Pages 31-43

  5. Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow In Vivo

    • Valérie Barbier, Ingrid G. Winkler, Robert Wadley, Jean-Pierre Lévesque
    Pages 45-63

  6. Analyzing Cell Death Events in Cultured Leukocytes

    • Karin Christenson, Fredrik B. Thorén, Johan Bylund
    Pages 65-86

  7. Towards a Four-Dimensional View of Neutrophils

    • Ben A. Croker, Andrew W. Roberts, Nicos A. Nicola
    Pages 87-99

  8. Isolation of Human and Mouse Neutrophils Ex Vivo and In Vitro

    • Yan Hu
    Pages 101-113

  9. Measurement of Oxidative Burst in Neutrophils

    • Yu Chen, Wolfgang G. Junger
    Pages 115-124

  10. Measurement of Neutrophil Elastase, Proteinase 3, and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic Substrates

    • Brice Korkmaz, Sylvie Attucci, Christophe Epinette, Elodie Pitois, Marie-Lise Jourdan, Luiz Juliano et al.
    Pages 125-138

  11. The Macrophage

    • Chris P. Verschoor, Alicja Puchta, Dawn M. E. Bowdish
    Pages 139-156

  12. Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice

    • R. Tedjo Sasmono, Elizabeth Williams
    Pages 157-176

  13. Generation of Mouse Bone Marrow-Derived Macrophages

    • Silvia Manzanero
    Pages 177-181

  14. Isolation and Differentiation of Monocytes–Macrophages from Human Blood

    • Dipti Vijayan
    Pages 183-187

  15. In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages

    • André Moraes Nicola, Arturo Casadevall
    Pages 189-197

  16. Measuring the Inflammasome

    • Olaf Groß
    Pages 199-222

  17. Arginine and Macrophage Activation

    • Mònica Comalada, Andree Yeramian, Manuel Modolell, Jorge Lloberas, Antonio Celada
    Pages 223-235

  18. Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation

    • Catherina H. Bird, Corrine Hitchen, Mark Prescott, Ian Harper, Phillip I. Bird
    Pages 237-250

  19. Detection of Human and Mouse Granzyme B Activity in Cell Extracts

    • Sarah Elizabeth Stewart, Matthew Stephen James Mangan, Phillip Ian Bird, Dion Kaiserman
    Pages 251-260

  20. T Cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T Cells in Chronic Intestinal Inflammation

    • Rajaraman Eri, Michael A. McGuckin, Robert Wadley
    Pages 261-275
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Editors and Affiliations

  • School of Dentistry, University of Queensland, Brisbane, Australia

    Robert B. Ashman

Bibliographic Information

  • Book Title: Leucocytes

  • Book Subtitle: Methods and Protocols

  • Editors: Robert B. Ashman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-527-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-526-8Published: 21 January 2012

  • Softcover ISBN: 978-1-4939-6231-0Published: 23 August 2016

  • eBook ISBN: 978-1-61779-527-5Published: 21 January 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 296

  • Topics: Cell Biology, Developmental Biology

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