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Rho GTPases pp 107–119Cite as

A Quantitative Fluorometric Approach for Measuring the Interaction of RhoGDI with Membranes and Rho GTPases

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 827))

Abstract

Tight regulation of Rho GTPase-signaling functions requires the proper localization of proteins to the membrane and cytosolic compartments, which can themselves undergo reconfiguration in response to signaling events. The importance of lipid-mediated membrane signal transduction continues to emerge as a critical event in many Rho GTPase-signaling pathways. Here we describe methods for the reconstitution of lipid-modified Rho GTPases with defined lipid vesicles and how this system can be used as a real-time assay for monitoring protein–membrane interactions.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY, USA

    Jared Johnson, Richard A. Cerione & Jon W. Erickson

  2. Department of Molecular Medicine, Cornell University, Ithaca, NY, USA

    Richard A. Cerione

Authors
  1. Jared Johnson
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  2. Richard A. Cerione
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  3. Jon W. Erickson
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Corresponding author

Correspondence to Richard A. Cerione .

Editor information

Editors and Affiliations

  1. Hull York Medical School, Centre for Biomedical Research, University of Hull, Cottingham Road, Hull, HU6 7RX, United Kingdom

    Francisco Rivero

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Johnson, J., Cerione, R.A., Erickson, J.W. (2012). A Quantitative Fluorometric Approach for Measuring the Interaction of RhoGDI with Membranes and Rho GTPases. In: Rivero, F. (eds) Rho GTPases. Methods in Molecular Biology, vol 827. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-442-1_8

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  • DOI: https://doi.org/10.1007/978-1-61779-442-1_8

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-61779-441-4

  • Online ISBN: 978-1-61779-442-1

  • eBook Packages: Springer Protocols

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