Abstract
Transcription factors are modular in nature in all organisms. In general, they have a DNA binding domain, one or more transcription activation and/or repressor domain, and often a dimerization domain. In many cases, transcription factors also have other protein–protein interaction domain(s). Mapping these functional domains in transcription factors is critical in understanding their molecular function. In this chapter, protocols for mapping the DNA binding domain and the transcription activation domain of a bHLH class of transcription factor are described. In principle, these protocols can be applied to other classes of transcription factors for mapping their functional domains.
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Acknowledgments
We thank Dr. Shen and members of the Huq laboratory for critical reading of this manuscript. This work was supported by grants from NSF (IOS-0822811 and IOS-0849287) to E.H.
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Zhu, L., Huq, E. (2011). Mapping Functional Domains of Transcription Factors. In: Yuan, L., Perry, S. (eds) Plant Transcription Factors. Methods in Molecular Biology, vol 754. Humana Press. https://doi.org/10.1007/978-1-61779-154-3_9
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DOI: https://doi.org/10.1007/978-1-61779-154-3_9
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