Abstract
The oomycete pathogen Hyaloperonospora arabidopsidis is a natural pathogen of Arabidopsis thaliana and a laboratory model for (1) understanding how Arabidopsis responds to pathogen attack; (2) comparative and functional genomics of oomycetes; and (3) the molecular basis and evolution of obligate biotrophy. Here, we describe procedures for propagation and long-term storage of H. arabidopsidis, which address complications arising from its biotrophic lifestyle that precludes growth on synthetic media. We also describe four assays that provide information on different facets of the H. arabidopsidis–Arabidopsis interaction.
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Acknowledgments
We thank Devdutta Deb for critical reading and the National Science Foundation for funding.
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McDowell, J.M., Hoff, T., Anderson, R.G., Deegan, D. (2011). Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis: A Model Oomycete Pathogen of Arabidopsis . In: McDowell, J. (eds) Plant Immunity. Methods in Molecular Biology, vol 712. Humana Press. https://doi.org/10.1007/978-1-61737-998-7_12
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DOI: https://doi.org/10.1007/978-1-61737-998-7_12
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