Plant Chromosome Engineering

1. Front Matter

   Pages i-xi

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2. Recent Advances in Plant Transformation

   • Shyamkumar Barampuram, Zhanyuan J. Zhang

   Pages 1-35

3. Engineering the Plastid Genome of Nicotiana sylvestris, a Diploid Model Species for Plastid Genetics

   • Pal Maliga, Zora Svab

   Pages 37-50

4. Homologous Recombination in Plants: An Antireview

   • Michal Lieberman-Lazarovich, Avraham A. Levy

   Pages 51-65

5. Chromosome Painting for Plant Biotechnology

   • Akio Kato, Jonathan C. Lamb, Patrice S. Albert, Tatiana Danilova, Fangpu Han, Zhi Gao et al.

   Pages 67-96

6. Plant B Chromosomes

   • Andreas Houben, Shuhei Nasuda, Takashi R. Endo

   Pages 97-111

7. Telomere Truncation in Plants

   • Chunhui Xu, Weichang Yu

   Pages 113-130

8. Engineered Plant Minichromosomes

   • Robert T. Gaeta, Lakshminarasimhan Krishnaswamy

   Pages 131-146

9. Method for Bxb1-Mediated Site-Specific Integration In Planta

   • Yuan-Yeu Yau, Yueju Wang, James G. Thomson, David W. Ow

   Pages 147-166

10. Targeted Mutagenesis in Arabidopsis Using Zinc-Finger Nucleases

    • Feng Zhang, Daniel F. Voytas

    Pages 167-177

11. Vectors and Methods for Hairpin RNA and Artificial microRNA-Mediated Gene Silencing in Plants

    • Andrew L. Eamens, Peter M. Waterhouse

    Pages 179-197

12. Recombinant Protein Expression in Nicotiana

    • Nobuyuki Matoba, Keith R. Davis, Kenneth E. Palmer

    Pages 199-219

13. Chromosome Analysis and Sorting Using Flow Cytometry

    • Jaroslav Doležel, Marie Kubaláková, Jarmila íhalíková, Pavla Suchánková, Hana Šimková

    Pages 221-238

14. Super-Stretched Pachytene Chromosomes for Plant Molecular Cytogenetic Mapping

    • Dal-Hoe Koo, Jiming Jiang

    Pages 239-245

15. Cytological Dissection of the Triticeae Chromosomes by the Gametocidal System

    • Takashi R. Endo

    Pages 247-257

16. Development and Use of Oat–Maize Chromosome Additions and Radiation Hybrids

    • Ralf G. Kynast, Oscar Riera-Lizarazu

    Pages 259-284

17. Enhancer Trapping in Plants

    • Sivanandan Chudalayandi

    Pages 285-300

18. Chromatin Beacons: Global Sampling of Chromatin Physical Properties Using Chromatin Charting Lines

    • Aniça Amini, Chongyuan Luo, Eric Lam

    Pages 301-314

19. Transposable Elements as Catalysts for Chromosome Rearrangements

    • Jianbo Zhang, Chuanhe Yu, Lakshminarasimhan Krishnaswamy, Thomas Peterson

    Pages 315-326

20. Back Matter

    Pages 327-329

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With an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocols, expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing the much needed novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals. This volume examines vital topics such as transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. As a part of the highly successful Methods in Molecular Biology™ series, the methodological chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and professional tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Chromosome Engineering: Methods and Protocols highlights the spectrum of tools currently available for modifying plant genomes and chromosomes and provides the foundation for crucial future developments.

• Agricultural improvement
• Chromosomal rearrangements
• Engineered minichromosomes
• Gene targeting and silencing
• Modified crops
• Mutagenesis
• Pharmaceuticals
• Plant species
• Protein expression
• Transformation procedures
• genetic engineering

• , Division of Biological Sciences, University of Missouri, Columbia, USA

  James A. Birchler

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