Abstract
MicroRNAs (miRNAs or miRs) are ∼22 nt single-stranded noncoding RNAs that control gene expression in eukaryotes. miRNAs play an essential role in all basic cellular processes including cell development, proliferation, differentiation, and apoptosis. Importantly, miRNAs regulate hematopoietic progenitor cells differentiation toward the different hematopoietic lineages. This occurs through the regulation of key factors involved in hematopoiesis (e.g., transcription factors, growth factor receptors). We, hereby, describe how to investigate the role of miRNAs in monocytopoiesis.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Orkin, S. H., and Zon, L. I. (2008) Hematopoiesis: an evolving paradigm for stem cell biology, Cell 132, 631–644.
Gordon, S., and Taylor, P. R. (2005) Monocyte and macrophage heterogeneity, Nat Rev Immunol 5, 953–964.
Fontana, L., Pelosi, E., Greco, P., Racanicchi, S., Testa, U., Liuzzi, F., Croce, C. M., Brunetti, E., Grignani, F., and Peschle, C. (2007) MicroRNAs 17-5p-20a-106a control monocytopoiesis through AML1 targeting and M-CSF receptor upregulation, Nat Cell Biol 9, 775–787.
Rosa, A., Ballarino, M., Sorrentino, A., Sthandier, O., De Angelis, F. G., Marchioni, M., Masella, B., Guarini, A., Fatica, A., Peschle, C., and Bozzoni, I. (2007) The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation, Proc Natl Acad Sci U S A 104, 19849–19854.
Bonci, D., Cittadini, A., Latronico, M. V., Borello, U., Aycock, J. K., Drusco, A., Innocenzi, A., Follenzi, A., Lavitrano, M., Monti, M. G., Ross, J., Jr., Naldini, L., Peschle, C., Cossu, G., and Condorelli, G. (2003) “Advanced” generation lentiviruses as efficient vectors for cardiomyocyte gene transduction in vitro and in vivo, Gene Ther 10, 630–636.
Naldini, L., Blomer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., and Trono, D. (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector, Science 272, 263–267.
Bahnson, A. B., Dunigan, J. T., Baysal, B. E., Mohney, T., Atchison, R. W., Nimgaonkar, M. T., Ball, E. D., and Barranger, J. A. (1995) Centrifugal enhancement of retroviral mediated gene transfer, J Virol Methods 54, 131–143.
Geronimi, F., Richard, E., Redonnet-Vernhet, I., Lamrissi-Garcia, I., Lalanne, M., Ged, C., Moreau-Gaudry, F., and De Verneuil, H. (2003) Highly efficient lentiviral gene transfer in CD34+ and CD34+/38−/lin- cells from mobilized peripheral blood after cytokine prestimulation, Stem Cells 21, 472–480.
Acknowledgments
This work was supported by the Italy–USA Oncology Program and the Biotechnology Program, Istituto Superiore di Sanità, and Associazione Italiana per la Ricerca sul Cancro (AIRC) to C.P.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Fontana, L., Sorrentino, A., Peschle, C. (2010). Regulation of Monocytopoiesis by MicroRNAs. In: Monticelli, S. (eds) MicroRNAs and the Immune System. Methods in Molecular Biology, vol 667. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-811-9_11
Download citation
DOI: https://doi.org/10.1007/978-1-60761-811-9_11
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-810-2
Online ISBN: 978-1-60761-811-9
eBook Packages: Springer Protocols