Overview
- Provides comprehensive methods and reviews to help investigators assess the potential of stem cells for myocardial repair and regeneration
- Presents guidelines for the reliable isolation of commonly assessed stem cells for myocardial repair
- Includes cutting-edge methods to determine engraftment, efficacy, lectrical integration, and safety
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 660)
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About this book
The feld of regenerative medicine is in its infancy state. Enthusiasm for the potential of organ regeneration lies with the potential pluripotency of stem cells to differentiate into various tissue types. This volume of Methods in Molecular Biology will focus on the use of stem cells for myocardial repair and regeneration. The emphasis of this issue will be to provide basic scientists, translational investigators, and cardiologists a means to evaluate the effcacy and safety of stem cells in a standardized fashion for myocardial regeneration. Many different cell types have been considered for myocardial repair. Adult card- myocytes are unable to survive even when transplanted into normal myocardium. The use of fetal or neonatal cardiomyocytes is not a feasible source of cells due to ethical concerns and donor availability. Therefore, the use of pluripotent stem cells has become the focus of a cell source for myocardial repair and regeneration. A variety of stem cell types have been suggested to participate in myocardial repair. This has led the investigators to search for the “optimal cell type for myocardial repair”. Reliable isolation of the cell source with the ability to expand the cell population is a prerequisite. In the frst section of this book, methods for isolation of commonly used stem cells being investigated for myocardial regeneration are presented. Once a stem cell source has been selected, the stem cell needs to be tested in an app- priate animal model before being translated into clinical practice.
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Table of contents (16 protocols)
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Front Matter
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Isolation and Characterization of Cells for Myocardial Regeneration
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Front Matter
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Stem Cells for Myocardial Repair and Regeneration: Where Are We Today?
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Isolation and Characterization of Cells for Myocardial Regeneration
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Front Matter
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Animal Models for Assessing Stem Cells for Myocardial Regeneration
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Front Matter
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Histological Assessment of Myocardial Regeneration
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Front Matter
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Measures of Cell Trafficking
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Front Matter
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Electrophysiology
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Front Matter
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Editors and Affiliations
Bibliographic Information
Book Title: Stem Cells for Myocardial Regeneration
Book Subtitle: Methods and Protocols
Editors: Randall J. Lee
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60761-705-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science + Business Media 2010
Hardcover ISBN: 978-1-60761-704-4Published: 09 August 2010
Softcover ISBN: 978-1-4939-6126-9Published: 23 August 2016
eBook ISBN: 978-1-60761-705-1Published: 03 August 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 267
Topics: Cardiology, Cell Biology, Stem Cells, Gene Therapy