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An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry

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Book cover Proteome Bioinformatics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 604))

Abstract

Protein quantification represents an important extension to identification proteomics, enabling the comparison of protein expression across different samples or treatments. Comparative protein quantification by mass spectrometry typically employs stable isotope incorporation, but recently, comparative quantification of label-free LCn-MS proteomics data has emerged as an alternative approach. In this chapter, we provide an overview of the different approaches for extracting quantitative data from label-free LCn-MS experiments. The computational procedure for recovering the quantitative information is outlined. Examples of statistical tests used to evaluate the relevance of results are also provided.

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Author information

Authors and Affiliations

  1. UNSW Cancer Research Centre, University of New South Wales, Sydney, NSW, Australia

    Jason W. H. Wong

  2. Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland

    Gerard Cagney

Authors
  1. Jason W. H. Wong
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  2. Gerard Cagney
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Editor information

Editors and Affiliations

  1. Fac. Life Sciences, University of Manchester, Oxford Rd., Manchester, M13 9PT, United Kingdom

    Simon J. Hubbard

  2. Fac. Veterinary Science, University of Liverpool, Liverpool, L69 7ZJ, United Kingdom

    Andrew R. Jones

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Wong, J.W.H., Cagney, G. (2010). An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry. In: Hubbard, S., Jones, A. (eds) Proteome Bioinformatics. Methods in Molecular Biology™, vol 604. Humana Press. https://doi.org/10.1007/978-1-60761-444-9_18

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  • DOI: https://doi.org/10.1007/978-1-60761-444-9_18

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-443-2

  • Online ISBN: 978-1-60761-444-9

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