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Meiosis

Volume 2, Cytological Methods

  • Book
  • © 2009

Overview

Editors:
  1. Scott Keeney

    1. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, U.S.A.

    View editor publications

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  • State-of-the-art techniques for analysis of meiotic chromosome dynamics
  • Comprehensive methods for studying meiosis in human and in a large number of model organisms including fungi, plants, invertebrate animals, zebrafish, and mice
  • Chapters on electron microscopy and conventional and immunofluorescent light microscopy
  • Techniques for enriching and culturing mouse meiotic cells
  • Techniques for spreading meiotic chromosomes and immunofluorescent detection of chromosome-associated proteins
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 558)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Cytological Analysis of Meiotic Chromosomes in Fungi

    1. Front Matter

      Pages 1-1
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    2. Chromosome Spreading and Immunofluorescence Methods in Saccharomyes cerevisiae

      • Beth Rockmill
      Pages 3-13

    3. Analysis of Schizosaccharomyces pombe Meiosis by Nuclear Spreading

      • Josef Loidl, Alexander Lorenz
      Pages 15-36

    4. Assaying Chromosome Pairing by FISH Analysis of Spread Saccharomyces cerevisiae Nuclei

      • Beth M. Weiner, Nancy Kleckner
      Pages 37-51

    5. Live-Cell Fluorescence Imaging of Meiotic Chromosome Dynamics in Schizosaccharomyces pombe

      • Haruhiko Asakawa, Yasushi Hiraoka
      Pages 53-64

    6. Time-Lapse Fluorescence Microscopy of Saccharomyces cerevisiae in Meiosis

      • Michael E. Dresser
      Pages 65-79

    7. Real-Time Imaging of Meiotic Chromosomes in Saccharomyces cerevisiae

      • Romain Koszul, Sei Kameoka, Beth M. Weiner
      Pages 81-89

    8. Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora

      • Denise Zickler
      Pages 91-114

    9. Meiotic Cytogenetics in Coprinus cinereus

      • Miriam E. Zolan, Patricia J. Pukkila
      Pages 115-127

  3. Cytological Analysis of Meiotic Chromosomes in Plants and Small Animals

    1. Front Matter

      Pages 129-129
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    2. Cytological Analysis of Arabidopsis thaliana Meiotic Chromosomes

      • Susan J. Armstrong, Eugenio Sanchez-Moran, F. Chris, H. Franklin
      Pages 131-145

    3. Electron Microscopic Immunogold Localization of Recombination-Related Proteins in Spreads of Synaptonemal Complexes From Tomato Microsporocytes

      • Stephen M. Stack, Lorinda K. Anderson
      Pages 147-169

    4. Cytological Analysis of Meiosis in Caenorhabditis elegans

      • Carolyn M. Phillips, Kent L. McDonald, Abby F. Dernburg
      Pages 171-195

    5. Cytological Analysis of Meiosis in Fixed Drosophila Ovaries

      • Kim S. McKim, Eric F. Joyce, Janet K. Jang
      Pages 197-216

    6. Analysis of Chromosome Dynamics and Chromosomal Proteins in Drosophila Spermatocytes

      • Sharon E. Thomas, Bruce D. McKee
      Pages 217-234

    7. Methods for Meiotic Chromosome Preparation, Immunofluorescence, and Fluorescence in situ Hybridization in Daphnia pulex

      • Dai Tsuchiya, Brian D. Eads, Miriam E. Zolan
      Pages 235-249

    8. Immunofluorescent Microscopic Study of Meiosis in Zebrafish

      • Nazafarin Kochakpour
      Pages 251-260

  4. Cytological and Histological Analysis of Mammalian Germ Cells and Meiotic Chromosomes

    1. Front Matter

      Pages 261-261
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    2. Staging of Mouse Seminiferous Tubule Cross-Sections

      • Emad A. Ahmed, Dirk G. de Rooij
      Pages 263-277
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Keywords

About this book

Each generation in a sexually reproducing organism such as a fly or a mouse passes through the bottleneck of meiosis, which is the specialized cell division that gives rise to haploid reproductive cells (sperm, eggs, spores, etc. ). The principal function of meiosis is to reduce the genome complement by half, which is accomplished through sequential execution of one round of DNA replication followed by two rounds of chromosome segregation. Within the extended prophase between DNA replication and the first meiotic division in most organisms, homologous maternal and paternal chromosomes pair with one another and undergo homologous recombination, which establishes physical connections that link the homologous chromosomes until the time they are separated at anaphase I. Recombination also serves to increase genetic diversity from one generation to the next by breaking up linkage groups. The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been an explosion of new information about how meiotic chromosomes pair, recombine, and are segregated. Progress has been driven by advances in three main areas: (1) genetic identification of meiosis-defective mutants and cloning of the genes involved; (2) development of direct physical assays for DNA intermediates and products of recombination; and (3) increasingly sophisticated cy- logical methods that describe chromosome behaviors and the spatial and temporal patterns by which specific proteins associate with meiotic chromosomes.

Editors and Affiliations

  • Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, U.S.A.

    Scott Keeney

Bibliographic Information

  • Book Title: Meiosis

  • Book Subtitle: Volume 2, Cytological Methods

  • Editors: Scott Keeney

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-103-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60761-102-8Published: 01 September 2009

  • Softcover ISBN: 978-1-4939-5835-1Published: 30 April 2017

  • eBook ISBN: 978-1-60761-103-5Published: 15 August 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 456

  • Number of Illustrations: 62 b/w illustrations, 12 illustrations in colour

  • Topics: Cell Biology, Microbiology, Eukaryotic Microbiology

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