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Arsonoliposomes: Preparation and Physicochemical Characterization

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Liposomes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 605))

Abstract

Arsonoliposomes (ARSL) which are liposomes that contain arsonolipids in their membranes have shown interesting anticancer and antiparasitic activity in vitro. Their lipid composition (the specific arsonolipids and/or phospholipids used for their preparation, and the relative amounts of each lipid type) highly influences their physicochemical properties as well as their in vivo kinetics and antiparasitic activity; however, their cytotoxicity towards cancer cells is minimally – if at all – modified. ARSL are prepared by a modification of the “one step” method followed or not by sonication (for formation of sonicated or non-sonicated ARSL, respectively). Arsonoliposomes may be composed only of arsonolipids (containing or not cholesterol) [plain ARSL], or they may contain mixtures of arsonolipids with phospholipids (with or without Chol) [mixed ARSL]. Herein, we describe in detail the preparation and physicochemical characterization of ARSL.

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Abbreviations

Ars:

Arsonolipid

ARSL:

Arsonoliposome

As:

Arsenic

C6:

Brain Glioma cell line (Rat)

CF:

5,6-Carboxyfluorescein

Chol:

Cholesterol

Cryo-EM:

Cryo electron microscopy

DMSO:

Dimethyl sulfoxide

DPPC:

1,2-Dipalmitoyl-sn-glyceroyl-3-phosphocholine

DPPE-PEG2000:

1,2-Dipalmitoyl-sn-glyceroyl-3-phosphoethanolamine conjugated to polyethylene glycol (MW 2000)

DSPC:

1,2-Distearoyl-sn-glyceroyl-3-phosphocholine

DSPE-PEG2000:

1,2-Distearoyl-sn-glyceroyl-3-phosphoethanolamine conjugated to polyethylene glycol (MW 2000)

EDTA:

Ethylenediamine tetraacetic acid

EM:

Electron microscopy

FCS:

Fetal calf serum

GH3:

Pituitary tumor cells

HL-60:

Human leukemia cell line

HUVEC:

Human umbilical vein endothelial cell

IC50:

50% Growth inhibition concentration

MEC:

Minimum effective concentration

NB4:

Human leukemia cell line

P:

Phosphorus

PBS:

Phosphate buffered saline

PC:

Phosphatidylcholine

PC3:

Prostate cancer cell line

PCS:

Photon correlation spectroscopy

PEG:

Polyethylene glycol

SUV:

Small unilamellar vesicles

TBS:

Tris-buffered saline

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Acknowledgments

Part of the work presented in this review was partly supported by the General Secretariat of Research and Technology, Athens Greece (PENED 95).

The authors would like to thank all the scientists that have contributed in this on-going research, and especially, Prof D. Fatouros, Prof. P. Loiseau, Prof. P. Frederik and Prof. P. Klepetsanis.

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Authors and Affiliations

  1. Laboratory of Pharmaceutical Technology, Department of Pharmacy, University of Patras, and FORTH – Institute of Chemical Engineering, Patras, Greece

    Sophia G. Antimisiaris

  2. Department of Chemistry, University of Patras, Patras, Greece

    Panayiotis V. Ioannou

Authors
  1. Sophia G. Antimisiaris
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  2. Panayiotis V. Ioannou
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Corresponding author

Correspondence to Sophia G. Antimisiaris .

Editor information

Editors and Affiliations

  1. College of Pharmacy, Midwestern University, N. 59th Avenue 19555, Glendale, 85308, U.S.A.

    Volkmar Weissig

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Antimisiaris, S.G., Ioannou, P.V. (2010). Arsonoliposomes: Preparation and Physicochemical Characterization. In: Weissig, V. (eds) Liposomes. Methods in Molecular Biology, vol 605. Humana Press. https://doi.org/10.1007/978-1-60327-360-2_10

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  • DOI: https://doi.org/10.1007/978-1-60327-360-2_10

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-359-6

  • Online ISBN: 978-1-60327-360-2

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