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Germline Stem Cells

  • Book
  • © 2008

Overview

Editors:
  1. Steven X. Hou

    1. National Cancer Institute, Frederick, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Shree Ram Singh

    1. National Cancer Institute, Frederick, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Readily reproducible protocols for the manipulation of germline stem cells in model organisms
  • State of the art methods optimized by leading experts in germline stem cells
  • Identification and regulation of germline stem cell in model organisms
  • Current techniques used in in vitro culture and application in germline stem cells
  • Presents sophisticated genetic, molecular and cellular techniques used in germline stem cell research
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 450)

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About this book

Stem cells are responsible for maintaining tissue homeostasis. They possess unlimited self-renewal potency. Through asymmetric cell division, a stem cell in adult tissues can produce an offspring that will maintain the stem cell populations and a daughter cell that will differentiate into various short-lived cell types to replace damaged or dying cells. Tumors may originate from a few transformed cancer stem cells. Stem cells have immense potential for therapeutic use in regenerative medicine and for developing anticancer therapies that specifically eliminate cancer stem cells. Germline stem cells are the source of human and animal reproduction. The knowledge gained from studying germline stem cells may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. To make use of this potential, we have to learn how to isolate, characterize, and maintain germline stem cells. To isolate, characterize, and maintain stem cells, we must first understand the molecular parameters that define a germline stem cell and the mechanisms that regulate stem cell behavior. The protocols in Germline Stem Cells are intended to present selected genetic, molecular, and cellular techniques used in germline stem cell research. The book is divided into two parts. Part I covers germline stem cell identification and regulation in model organisms. Part II covers current techniques used in in vitro culture and applications of germline stem cells.

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xii
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  2. Identification and Regulation of Germline Stem Cells in Model Organisms

    1. The Development of Germline Stem Cells in Drosophila

      • David A. Dansereau, Paul Lasko
      Pages 3-26

    2. Analysis of the C. elegans Germline Stem Cell Region

      • Sarah L. Crittenden, Judith Kimble
      Pages 27-44

    3. Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell

      • Shree Ram Singh, Steven X. Hou
      Pages 45-59

    4. Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells

      • Wei Liu, Steven X. Hou
      Pages 61-70

    5. Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (Milkweed Bug Oncopeltus fasciatus)

      • David C. Dorn, August Dorn
      Pages 71-94

    6. High-Resolution Light Microscopic Characterization of Spermatogonia

      • Hélio Chiarini-Garcia, Marvin L. Meistrich
      Pages 95-107

    7. Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes

      • Jens Ehmcke, Stefan Schlatt
      Pages 109-118

    8. Epigenetic Control in Male Germ Cells

      • Durga Prasad Mishra, Paolo Sassone-Corsi
      Pages 119-126

    9. GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro

      • Hannu Sariola, Tiina Immonen
      Pages 127-135

  3. In Vitro Culture and Applications of Germline Stem Cells

    1. Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts

      • Ina Dobrinski, Rahul Rathi
      Pages 139-148

    2. Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in Mice

      • Derek J. McLean
      Pages 149-162

    3. Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture

      • F. Kent Hamra, Karen M. Chapman, Zhuoru Wu, David L. Garbers
      Pages 163-179

    4. Deriving Mouse Spermatogonial Stem Cell Lines

      • Ilaria Falciatori, Kate Lillard-Wetherell, Zhuoru Wu, F. Kent Hamra, David L. Garbers
      Pages 181-192

    5. Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes

      • Christina Tenenhaus Dann, David L. Garbers
      Pages 193-209

    6. Testicular Germ Cell Tumors in Mice

      • Jason D. Heaney, Joseph H. Nadeau
      Pages 211-231

    7. Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries

      • Antonin Bukovsky, Satish K. Gupta, Irma Virant-Klun, Nirmala B. Upadhyaya, Pleas Copas, Stuart E. Van Meter et al.
      Pages 233-265

  4. Back Matter

    Pages 673-677
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Reviews

From the reviews:

"This book provides background information and protocols for identifying, isolating, characterizing, and manipulating … germline stem cells. … This book is written for basic science and clinical researchers … as well as those interested in development and reproductive biology. Biomedical scientists and clinicians … will also appreciate the wealth of new information. … This is an outstanding contribution to the literature in germ cell biology. ... will be valued by all those interested in development of the germ lineage and stem cell biology." (Bruce A. Fenderson, Doody’s Review Service, October, 2008)

Editors and Affiliations

  • National Cancer Institute, Frederick, USA

    Steven X. Hou, Shree Ram Singh

Bibliographic Information

  • Book Title: Germline Stem Cells

  • Editors: Steven X. Hou, Shree Ram Singh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-214-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-60327-213-1Published: 07 March 2008

  • Softcover ISBN: 978-1-61737-880-5Published: 15 October 2014

  • eBook ISBN: 978-1-60327-214-8Published: 14 March 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 273

  • Topics: Cell Biology, Molecular Medicine, Developmental Biology, Human Genetics

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