Abstract
As interest in lytic phages as antimicrobial therapies or as treatments to reduce environmental contamination with pathogenic bacteria has increased, so has the need to determine if the use of lytic phages may lead to dissemination of virulence factors through generalized transduction, as occurs with temperate phages. Here we describe simple methods we have developed to determine if a lytic phage, rV5, can mediate generalized transduction in Escherichia coli O157:H7. These sensitive methods can be easily adapted to study generalized transduction between virulent and avirulent strains of bacteria.
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References
Petty, N.K., I.J. Foulds, E. Pradel, J.J. Ewbank, G.P. Salmond, N.K. Petty, I.J. Foulds, E. Pradel, et al. 2006. A generalized transducing phage (fIF3) for the genomically sequenced Serratia marcescens strain Db11: a tool for functional genomics of an opportunistic human pathogen. Microbiology 152: 1701–1708.
Bendig, M.M. and H. Drexler. 1977. Transduction of bacteriophage Mu by bacteriophage T1. Journal of Virology 22:640–645.
Drexler, H. 1977. Specialized transduction of the biotin region of Escherichia coli by phage T1. Molecular & General Genetics 152:59–63.
Drexler, H. 1970. Transduction by bacteriophage T1. Proceedings of the National Academy of Sciences of the United States of America 66:1083–1088.
Roberts, M.D. and H. Drexler. 1981. Isolation and genetic characterization of T1-transducing mutants with increased transduction frequency. Virology 112: 662–669.
Wilson, G.G., K.Y. Young, G.J. Edlin, W. Konigsberg, G.G. Wilson, K.Y. Young, G.J. Edlin, and W. Konigsberg. 1979. High-frequency generalised transduction by bacteriophage T4. Nature 280:80–82.
Young, K.K., G.J. Edlin, and G.G. Wilson. 1982. Genetic analysis of bacteriophage T4 transducing bacteriophages. Journal of Virology 41:345–347.
Tianiashin, V.I., V.I. Zimin, A.M. Boronin, V.I. Tianiashin, V.I. Zimin, and A.M. Boronin. 2003. The cotransduction of pET system plasmids by mutants of T4 and RB43 bacteriophages. Mikrobiologiia 72:785–791.
Taniashin, V.I., A.A. Zimin, M.G. Shliapnikov, A.M. Boronin, V.I. Taniashin, A.A. Zimin, M.G. Shliapnikov, and A.M. Boronin. 2003. Transduction of plasmid antibiotic resistance determinants with pseudo-T-even bacteriophages. Genetika 39:914–926.
Cerquetti, M.C., A.M. Hooke, M.C. Cerquetti, and A.M. Hooke. 1993. Vi I typing phage for generalized transduction of Salmonella typhi. Journal of Bacteriology 175:5294–5296.
Dzhusupova, A.B., T.G. Plotnikova, V.N. Krylov, A.B. Dzhusupova, T.G. Plotnikova, and V.N. Krylov. 1982. Detection of transduction by virulent bacteriophage f KZ of Pseudomonas aeruginosa chromosomal markers in the presence of plasmid RMS148. Genetika 18:1799–1802.
Morgan, A.F. 1979. Transduction of Pseudomonas aeruginosa with a mutant of bacteriophage E79. Journal of Bacteriology 139: 137–140.
Gorbunova, S.A., V.S. Akhverdian, L.V. Cheremukhina, V.N. Krylov, S.A. Gorbunova, V.S. Akhverdian, L.V. Cheremukhina, and V.N. Krylov. 1985. Effective method of transduction with virulent phage pf16 using specific mutants of Pseudomonas putida PpG1. Genetika 21:872–874.
Daz, R., T.G. De, J.L. Canovas, R. Daz, G. De Torrontegui, and J.L. Canovas. 1976. Generalized transduction of Pseudomonas putida with a thermosensitive mutant of phage pf16h2. Microbiologia Espanola 29:33–45.
Rheinwald, J.G., A.M. Chakrabarty, and I.C. Gunsalus. 1973. A transmissible plasmid controlling camphor oxidation in Pseudomonas putida. Proceedings of the National Academy of Sciences of the United States of America 70:885–889.
Weiss, B.D., M.A. Capage, M. Kessel, and S.A. Benson. 1994. Isolation and characterization of a generalized transducing phage for Xanthomonas campestris pv. campestris. Journal of Bacteriology 176:3354–3359.
Meile, L., P. Abendschein, T. Leisinger, L. Meile, P. Abendschein, and T. Leisinger. 1990. Transduction in the archaebacterium Methanobacterium thermoautotrophicum Marburg. Journal of Bacteriology 172: 3507–3508.
Bender, R.A. 1981. Improved generalized transducing bacteriophage for Caulobacter crescentus. Journal of Bacteriology 148: 734–735.
Petty, N.K., A.L. Toribio, D. Goulding, I. Foulds, N. Thomson, G. Dougan, and G.P. Salmond. 2007. A generalized transducing phage for the murine pathogen Citrobacter rodentium. Microbiology 153:2984–2988.
Canosi, U., G. Luder, and T.A. Trautner. 1982. SPP1-mediated plasmid transduction. Journal of Virology 44:431–436.
de Lencastre, H. and L.J. Archer. 1980. Characterization of bacteriophage SPP1 transducing particles. Journal of General Microbiology 117:347–355.
Riska, P.F., Y. Su, S. Bardarov, L. Freundlich, G. Sarkis, G. Hatfull, C. Carriere, V. Kumar, et al. 1999. Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis strains by using a luciferase reporter phage and the Bronx Box. Journal of Clinical Microbiology 37:1144–1149.
Waddell, T.E., A. Mazzocco, J. Pacan, R. Johnson, R. Ahmed, C. Poppe, and C. Khakhria. 2002. Use of bacteriophages to control Escherichia coli O157 infections in cattle, United States Patent No. 6,485,902.
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Waddell, T.E., Franklin, K., Mazzocco, A., Kropinski, A.M., Johnson, R.P. (2009). Generalized Transduction by Lytic Bacteriophages. In: Clokie, M.R., Kropinski, A.M. (eds) Bacteriophages. Methods in Molecular Biology™, vol 501. Humana Press. https://doi.org/10.1007/978-1-60327-164-6_25
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DOI: https://doi.org/10.1007/978-1-60327-164-6_25
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