Abstract
Bromeliads are tropical plants that are native to the Americas with a wide distribution in the rain forests, deserts and coastal areas. They are mostly epiphytes and terrestrial, diverse and important from the ecological point of view, they are found in microhabitats in strong interactions with fauna. Most of the ecosystems where they naturally occur are now endangered. Bromeliads are also one of the bases of the ornamental industry worldwide, being commercialized according to the features and colour of the foliage and flowers. This industry relies also on breeding programmes generating new hybrid bromeliads with improved bloom and foliage. Thus, advanced propagation systems based on micropropagation are valuable tools for both bromeliad germplasm conservation and for the mass clonal propagation of a selected genotype. In this chapter, a series of in vitro regenerative protocols for bromeliad mass propagation are described in detail. Emphasis is placed on the Brazilian native species, mainly those from the Atlantic Forest biome. The morphogenetic pathway based on nodule cluster culture is stressed, as (a) it seems to be recurrent in bromeliads, (b) it results in high regenerative rates, and (c) it is easy to handle. Protocols based on somatic embryogenesis and encapsulated units using hydrogel are presented. Finally, the use of temporary immersion systems (TIS) for mass propagation is described in detail for two bromeliads: Aechmea fasciata, and Vriesea brusquensis.
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References
Martinelli G (2000) The Bromeliads of the Atlantic forest. Sci Am 282:86–93
Baensch U, Baensch U (1994) Blooming Bromeliads. Rasch Druck Verlag, Bramsche, GE
Reitz R (1983) Bromeliáceas e a malária – bromélia endêmica. Flora ilustrada Catarinense, Itajaí
Benzing DH (2000) Bromeliaceae: profile of an adaptive radiation. Cambridge University Press, Cambridge
Smith LB, Downs RJ (1977)Tillandsioideae (Bromeliaceae). Flora Neotrop. Hafner Press, New York
Alves GM, Rech Filho A, Puchalski A, Reis MS, Nodari RO, Guerra MP (2004) Allozymic markers and genetic characterization of a natural population of Vriesea friburgensis var. paludosa, a bromeliad from the Atlantic Forest. P. Genet Res 2:23–28
Rech Filho A, Dal Vesco LL, Nodari RO, Lischka RW, Muller CV, Guerra MP (2005) Tissue culture for the conservation and mass propagation of Vriesea reitzii Leme and Costa, a bromeliad threatened of extinction from the Brazilian Atlantic Forest. Biodivers Conserv 14:1799–1808
Mercier H, Kerbauy GB (1992) In vitro multiplication of Vriesea fosteriana. Plant Cell Tissue Organ Cult 30:247–249
Mercier H, Kerbauy GB (1993) Micro-propagation of Dychia macedoi – an endangered endemic Brazilian bromeliad. Bot Gard Microprop News 1:70–72
Vinterhalter B, Vinterhalter D (1994) True-to-the type in vitro propagation of Aechmea fasciata Baker. Sci Hortic 57:253–263
Daquinta M, Almeida AP, Guerra MP (1998) In vitro morphogenesis of immature flower and buds of flower stalk in Dyckia distachya. J Bromeliad Soc 49:72–76
Carneiro LA, Cândido MSD, Araujo RFG, Fonseca MHPB, Crocomo OJ, Mansur E (1998) Clonal propagation of Cryptanthus sinuosos L.B. Smith, an endemic stoloniferous Bromeliaceae species from Rio de Janeiro, Brazil. Plant Tissue Cult Biotechnol 4:153–158
George EF (1993) Plant propagation by tissue culture: the technology. Exegetics, Edington
Pompelli MF, Guerra MP (2005) Micropro-pagation enables the mass propagation and conservation of Dyckia distachya Hassler.Crop Breeding Apl Biotechnol 5:117–126
Hosoki T, Asahira T (1980) In vitro propagation of bromeliads in liquid culture. HortScience 15:603–604
Mercier H, Kerbauy GB (1997) Micropropa-gation of ornamental bromeliads (Bromeliaceae). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Springer, Berlin, pp 43–57
Carneiro LA, Araujo RFG, Brito GJM, Fonseca MHPB, Costa A, Crocomo OJ, Mansur E (1999) In vitro regeneration from leaf explants of Neoregelia cruenta (R. Graham) L.B. Smith, an endemic bromeliad from Eastern Brazil. Plant Cell Tissue Organ Cult 55:79–83
Alves GM, Guerra MP (2001) Micropropagation for mass propagation and conservation of Vriesea friburgensis var. paludosa from microbuds. J Bromeliad Soc 51:202–212
Pompelli MF, Fernandes D, Guerra MP (2005) Somatic embryogenesis in Dyckia distachia Hassler (Bromeliaceae) - An endangered bromeliad from South Brazil. Propagation Ornament Plants 5:192–198
Alves GM, Dal Vesco LL, Guerra MP (2006) Micropropagation of the Brazilian endemic bromeliad Vriesea reitzii trough nodule clusters culture. Sci Hortic 110:204–207
Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue cultures. Physiol Plant 15:473–497
Morel GM, Wetmore RH (1951) Tissue culture of monocotyledons. Am J Bot 38:138–140
Feuser S, Nodari RO, Guerra MP (2001) Comparative efficiency of stationary culture and temporary immersion systems for pineapple micropropagation. R Bras Frutic 23:6–10
Klein RM (1990) Espécies raras ou ameaçadas de extinção – Estado de Santa Catarina: mirtáceas e bromeliáceas. IBGE, Rio de Janeiro
Leme EMV, Costa A (1991) A new species from Southern Brazil, a tribute to Father Raulino Reitz. J. Bromeliad Soc 41:195–198
Smith LB, Downs RJ (1974) Bromeliaceae (Pitcairnioideae). Flora Neotr. New York, Hafner Press
Etienne H, Berthouly M (2002) Temporary immersion systems in plant micropropagation. Plant Cell Tissue Organ Cult 69:215–231
Dal Vesco LL, Pinto AA, Zaffari GR, Nodari RO, Reis MS, Guerra MP (2001) Improving pineapple micropropagation protocol through explant size and medium composition manipulation. Fruits 56:143–154
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The authors gratefully acknowledge the financial support of CAPES, CNPq, FAPESC, and FAPESP for the grants and fellowships.
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Guerra, M.P., Vesco, L.L.D. (2010). Strategies for the Micropropagation of Bromeliads. In: Jain, S., Ochatt, S. (eds) Protocols for In Vitro Propagation of Ornamental Plants. Methods in Molecular Biology, vol 589. Humana Press. https://doi.org/10.1007/978-1-60327-114-1_6
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DOI: https://doi.org/10.1007/978-1-60327-114-1_6
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