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Profiling Genome-Wide Histone Modifications and Variants by ChIP-chip on Tiling Microarrays in S. cerevisiae

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DNA-Protein Interactions

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 543))

Summary

Chromatin immunoprecipitation coupled to DNA microarray has become a widely used method to study transcription factors and chromatin structure. Here, we provide a detailed protocol for the localization of the variant histone Htz1 in the S. cerevisiae genome. This protocol can easily be adapted to fit other purposes such as profiling histone modifications.

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Correspondence to François Robert .

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Bataille, A.R., Robert, F. (2009). Profiling Genome-Wide Histone Modifications and Variants by ChIP-chip on Tiling Microarrays in S. cerevisiae . In: Leblanc, B., Moss, T. (eds) DNA-Protein Interactions. Methods in Molecular Biology™, vol 543. Humana Press. https://doi.org/10.1007/978-1-60327-015-1_18

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  • DOI: https://doi.org/10.1007/978-1-60327-015-1_18

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-014-4

  • Online ISBN: 978-1-60327-015-1

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