Abstract
In this chapter we present the methods developed in our laboratory for in vivo imaging of oligonucleotidic aptamers. These methods relate to (i) the labelling of aptamers with fluorine-18, a positron emitter, (ii) Positron Emission Tomography imaging of laboratory animals with [18F]aptamers and (iii) labelling with fluorescent dyes and optical imaging of aptamers in mice.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ellington, A.D. and Szostak, J.W. (1990) In vitro selection of RNA molecules that bind specific ligands. Nature 346, 818–822.
Tuerk, C. and Gold, L. (1990) Systematic evolution of ligands by exponential enrichment : RNA ligands to bacteriophage T4 DNA polymerase. Science 249, 505–510.
Blank, M., Weinschenk, T., Priemer, M. and Schluesener, H. (2001) Systematic evolution of a DNA aptamer binding to rat brain tumor microvessels. Selective targeting of endothelial regulatory protein pigpen. J. Biol. Chem. 276, 16464–16468.
Hicke, B.J., Marion, C., Chang, Y.F., Gould, T., Lynott, C.K., Parma, D., Schmidt, P.G. and Warren, S. (2001) Tenascin-C aptamers are generated using tumor cells and purified protein. J. Biol. Chem. 276, 48644–48654.
Daniels, D.A., Chen, H., Hicke, B.J., Swiderek, K.M. and Gold, L. (2003) A tenascin-C aptamer identified by tumor cell SELEX: systematic evolution of ligands by exponential enrichment. Proc. Natl. Acad. Sci. U S A, 100, 15416–15421.
Cerchia, L., Duconge, F., Pestourie, C., Boulay, J., Aissouni, Y., Gombert, K., Tavitian, B., Franciscis, V.D. and Libri, D. (2005) Neutralizing aptamers from whole-Cell SELEX inhibit the RET receptor tyrosine kinase. PLoS Biol. 3, e123.
Pestourie, C., Tavitian, B. and Duconge, F. (2005) Aptamers against extracellular targets for in vivo applications. Biochimie 87, 921–930.
Cerchia, L. and de Franciscis, V. (2007) Nucleic acid-based aptamers as promising therapeutics in neoplastic diseases. Methods Mol. Biol. 361:187–200.
Ntziachristos, V., Leroy-Willig, A. and Tavitian, B. (Eds.) (2007) Textbook ofIn Vivo Imaging in Vertebrates. John Wiley & Sons, Chichester.
Boisgard, R., Kuhnast, B., Vonhoff, S., Younes, C., Hinnen, F., Verbavatz, J.M., Rousseau, B., Fürste, J.P., Wlotzka, B., Dollé, F., Klussmann, S. and Tavitian, B. (2004) In vivo biodistribution and pharmacokinetics of [18F]-labelled Spiegelmers: a new class of oligonucleotidic radiopharmaceuticals. Eur. J. Nucl. Med. Mol. Imaging 32, 470–477.
Younes, C.K., Boisgard, R. and Tavitian, B. (2002) Labelled oligonucleotides as radiopharmaceuticals: pitfalls, problems and perspectives. Curr. Pharm. Des. 8, 1451–1466.
Tavitian, B., Terrazzino, S., Kuhnast, B., Marzabal, S., Stettler, O., Dolle, F., Deverre, J.R., Jobert, A., Hinnen, F., Bendriem, B., Crouzel, C. and Di Giamberardino, L. (1998) In vivo imaging of oligonucleotides with positron emission tomography. Nat Med. 4, 467–71.
Tavitian, B. (2003) In vivo imaging with oligonucleotides for diagnosis and drug development. Gut 52, 1–7.
Lucignani, G. (2006) Aptamers and in-beam PET for advanced diagnosis and therapy optimisation. Eur. J. Nucl. Med. Mol. Imaging 33, 1095–1097.
Perkins, A.C. and Missailidis, S. (2007) Radiolabelled aptamers for tumour imaging and therapy. Q. J. Nucl. Med. Mol. Imaging 51, 292–296.
Charlton, J., Sennello, J. and Smith, D. (1997) In vivo imaging of inflammation using an aptamer inhibitor of human neutrophil elastase. Chem. Biol. 4, 809–16.
Hicke, B.J., Stephens, A.W., Gould, T., Chang, Y.F., Lynott, C.K., Heil, J., Borkowski, S., Hilger, C.S., Cook, G., Warren, S. and Schmidt, P.G. (2006) Tumor targeting by an aptamer. J. Nucl. Med. 47, 668–678.
Kuhnast, B., de Bruin, B., Hinnen, F., Tavitian, B. and Dollé, F. (2004) Design and synthesis of a new [18F]fluoropyridine-based haloacetamide reagent for the labeling of oligonucleotides: 2-bromo-N-[3-(2-[18F]fluoro-pyridin-3-yloxy)-propyl]-acetamide. Bioconj. Chem. 15, 617–627.
de Bruin, B., Kuhnast, B., Hinnen, F., Yaouancq, L., Amessou, M., Johannes, L., Samson, A., Boisgard, R., Tavitian, B. and Dollé, F. (2005) 1-[3-(2-[18F]Fluoropyridin-3-yloxy)propyl]pyrrole-2,5-dione: Design, synthesis and radiosynthesis of a new [18F]fluoropyridine-based maleimide reagent for the labeling of peptides and proteins. Bioconj. Chem. 16, 406–420.
Dollé, F. Preparation of[18F]-labeled maleimides, their use for marking macromolecules for medical imaging. WO2004002984.
Dolci, L., Dollé, F., Jubeau, S., Vaufrey, F. and Crouzel, C. (1999) 2-[18F]fluoropyridines by no-carrier-added nucleophilic aromatic substitution with [18F]FK-K222 – a comparative study. J. Labelled Comp. Radiopharm. 42, 975–985
Karramkam, M., Hinnen, F., Vaufrey, F. and Dollé. F. (2003) 2-, 3- and 4-[18F]fluoropyridine by no-carrier-added nucleophilic aromatic substitution with K[18F]F-K222 – a comparative study. J. Labelled Comp. Radiopharm. 46, 979–992.
Dollé, F. (2005) Fluorine-18-labelled fluoropyridines: advances in radiopharmaceutical design. Curr. Pharm. Design, 11, 3221–3235.
Dollé. F. (2007) [18F]fluoropyridines: from conventional radiotracers to the labelling of macromolecules such as proteins and oligonucleotides. In: PET Chemistry: The Driving Force in Molecular Imaging, Ernst Schering Research Foundation Ed., Springer Verlag, Berlin: Heidelberg, 62, 113–157.
Friebe, M., Dollé, F., Kuhnast, B., Hinnen, F., Boisgard, R., Tavitian, B., Dinkelborg, L. and Hecht, M. 18F-labelled aptamers. EP07075801.
Graves, E.E., Yessayan, D., Turner, G., Weissleder, R., and Ntziachristos, V. (2005) Validation of in vivo fluorochrome concentrations measured using fluorescence molecular tomography. J. Biomed. Opt. 10, 44019 .
Maroy, R., Boisgard,R., Comtat, C., Frouin, V., Cathier, P., Duchesnay, E., Dollé, F., Nielsen, P., Trébossen, R., and Tavitian, B. (2008) An unsupervised method for segmentation of rodent whole-body dynamic PET images based on voxel dynamics. IEEE Trans. Med. Imaging, in press.
Acknowledgements
Studies relating to in vivo imaging of aptamers in our laboratory are supported by the EMIL and DIMI European networks, the Agence Nationale de la Recherche, the Institut National du Cancer, the Cancéropôle Ile de France, the Fondation de France and the Association pour la Recherche sur le Cancer.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Tavitian, B., Ducongé, F., Boisgard, R., Dollé, F. (2009). In Vivo Imaging of Oligonucleotidic Aptamers. In: Mayer, G. (eds) Nucleic Acid and Peptide Aptamers. Methods in Molecular Biology™, vol 535. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-557-2_15
Download citation
DOI: https://doi.org/10.1007/978-1-59745-557-2_15
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-934115-89-3
Online ISBN: 978-1-59745-557-2
eBook Packages: Springer Protocols