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Protein Targeting Protocols

  • Book
  • © 2007
  • Latest edition

Overview

Editors:
  1. Mark Giezen

    1. School of Biological and Chemical Sciences, Queen Mary, University of London, UK

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  • Thorough and state-of-the-art update of the successful first edition
  • Emphasis placed on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems
  • Targeting protocols from animal, plant, fungal, and protist systems
  • Chapters written by world leaders in the field

Part of the book series: Methods in Molecular Biology (MIMB, volume 390)

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About this book

In this highly anticipated update of the extremely successful Protein Targeting Protocols, experts from around the world provide the latest protocols on for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Unlike the first edition, however, this volume places emphasis on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems.

Authors provide state-of-the-art information on the fast moving fields of import machinery of mitochondria and plastids, as well as a detailed protocol on the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching. Bacterial protein targeting protocols using the Sec-system, type-V secretion apparatus, and the Tat-pathway are described, as is a periplasmic targeting protocol. Chapters also include bioinformatics methods to guide readers through the ever-increasing number of in silico tools currently available. Editor Mark van der Giezen has succeeded in including targeting protocols from different systems, including animal, plant, fungal, and protist models, providing insights into the intricate challenges presented by these diverse systems.

Authoritative and state-of-the-art, Protein Targeting Protocols, Second Edition, provides useful techniques that will aid scientists working with a variety of organisms.

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Keywords

Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
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  2. Bacterial Systems

    1. Front Matter

      Pages 1-1
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    2. Localization and Mobility of Bacterial Proteins by Confocal Microscopy and Fluorescence Recovery After Photobleaching

      • Conrad W. Mullineaux
      Pages 3-16

    3. Membrane Protein Insertion and Secretion in Bacteria

      • Jeanine de Keyzer, Martin van der Laan, Arnold J.M. Driessen
      Pages 17-32

    4. Identification of Autotransporter Proteins Secreted by Type V Secretion Systems in Gram-Negative Bacteria

      • Yihfen T. Yen, Christos Stathopoulos
      Pages 33-46

    5. Periplasmic Targeting of Recombinant Proteins in Escherichia coli

      • Filipe J. Mergulhão, Gabriel A. Monteiro
      Pages 47-61

    6. In Vitro Analysis of the Bacterial Twin-Arginine-Dependent Protein Export

      • Michael Moser, Sascha Panahandeh, Eva Holzapfel, Matthias Müller
      Pages 63-79

  3. Eukaryotic Systems

    1. Front Matter

      Pages 81-81
      Download chapter PDF

    2. Nuclear Import Properties of the Sex-Determining Factor SRY

      • Jade K. Forwood, Gurpreet Kaur, David A. Jans
      Pages 83-97

    3. The Mitochondrial Machinery for Import of Precursor Proteins

      • Kipros Gabriel, Nikolaus Pfanner
      Pages 99-117

    4. A Genetic Method to Identify Mitochondrial Proteins in Living Mammalian Cells

      • Takeaki Ozawa, Yoshio Umezawa
      Pages 119-130

    5. In Vitro and In Vivo Methods to Study Protein Import Into Plant Mitochondria

      • Shashi Bhushan, Pavel F. Pavlov, Charlotta Rudhe, Elzbieta Glaser
      Pages 131-150

    6. Protein Targeting to Mitochondria of Saccharomyces cerevisiae and Neurospora crassa

      • Panagiotis Papatheodorou, Grażyna Domańska, Joachim Rassow
      Pages 151-166

    7. A Mitosome Purification Protocol Based on Percoll Density Gradients and Its Use in Validating the Mitosomal Nature of Entamoeba histolytica Mitochondrial Hsp70

      • Jorge Tovar, Siân S. E. Cox, Mark van der Giezen
      Pages 167-177

    8. The Chloroplast Protein Import Machinery: A Review

      • Friederike Hörmann, Jürgen Soll, Bettina Bölter
      Pages 179-193

    9. Import of Plastid Precursor Proteins Into Pea Chloroplasts

      • Bettina Bölter, Jürgen Soll
      Pages 195-206

    10. Protein Targeting in ‘‘Secondary’’ or ‘‘Complex’’ Chloroplasts

      • Balbir K. Chaal, Beverley R. Green
      Pages 207-217

    11. Protein Trafficking to the Complex Chloroplasts of Euglena

      • Rostislav Vacula, Silvia Slàvikovà, Steven D. Schwartzbach
      Pages 219-237

    12. Fluorescent Protein Fusions for Protein Localization in Plants

      • John Runions, Chis Hawes, Smita Kurup
      Pages 239-256

    13. Genetic Transformation

      • Peter G. Kroth
      Pages 257-267
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Editors and Affiliations

  • School of Biological and Chemical Sciences, Queen Mary, University of London, UK

    Mark Giezen

Bibliographic Information

  • Book Title: Protein Targeting Protocols

  • Editors: Mark Giezen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-466-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-702-0Published: 21 May 2007

  • Softcover ISBN: 978-1-61737-727-3Published: 19 November 2010

  • eBook ISBN: 978-1-59745-466-7Published: 01 April 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 501

  • Topics: Protein Science, Cell Biology, Human Genetics

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