Overview
- Provides an easily accessible reference volume for epitope mapping written by leading researchers in the field
- Details the process of turning epitope mapping into biomarker discovery
- Covers a wide range of antibody-based and T-cell-based assay formats and systems
- Presents a modular approach to experimental design, screening modalities, detection technologies, and approaches to data analysis
- Comprehensive guide to developing, running, and analyzing B- and T-cell-based assays
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 524)
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About this book
Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.
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Keywords
Table of contents (32 protocols)
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Front Matter
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B-cell Epitope Mapping
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Front Matter
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Whole Antigen Methods
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Front Matter
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Peptide Library Approaches
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Front Matter
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Reviews
From the reviews of the second edition:
"This is a beautifully presented and very well-written book on the subject … . The diagrams and data are of a very high standard and black and white … . a fantastic lab resource and would benefit any immunology lab where epitope mapping is being or will be used. … a useful reference book of interest to staff from Pls to PhD students, providing both a thorough background and working methods. … a high quality and reader friendly book and I would highly recommend it." (Barbara Guinn, Immunology News, August, 2009)
Bibliographic Information
Book Title: Epitope Mapping Protocols
Editors: Mike Schutkowski, Ulrich Reineke
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-450-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2009
Hardcover ISBN: 978-1-934115-17-6Published: 20 May 2009
Softcover ISBN: 978-1-61737-924-6Published: 20 January 2011
eBook ISBN: 978-1-59745-450-6Published: 20 April 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 450
Number of Illustrations: 66 b/w illustrations, 19 illustrations in colour
Topics: Biochemistry, general, Immunology, Antibodies